| Title: | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
| Author(s): | Morita S; Horii T; Kimura M; Kobayashi R; Tanaka H; Akita H; Hatada I; |
| Address: | "Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi 371-8512, Japan. Laboratory of DDS Design and Drug Disposition, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan. Viral Vector Core, Gunma University Initiative for Advanced Research (GIAR), 3-39-15 Showa-machi, Maebashi 371-8511, Japan" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Knockout mice are useful tools that can provide information about the normal function of genes, including their biochemical, developmental, and physiological roles. One problem associated with the generation of knockout mice is that the loss of some genes of interest produces a lethal phenotype. Therefore, the use of conditioned knockout mice, in which genes are disrupted in specific organs, is essential for the elucidation of disease pathogenesis and the verification of drug targets. In general, conditional knockout mice are produced using the Cre/loxP system; however, the production of the large numbers of Cre/flox knockout and control mice required for analysis requires substantial time and effort. Here, we describe the generation of liver-specific conditional knockout mice via the introduction of lipid nanoparticles encapsulating Cre mRNA into the liver of floxed mice. This technique does not require the production of offspring by mating floxed mice and is therefore more convenient than the conventional method. The results presented here demonstrate that the LNP-based method enables liver-specific gene knockout in a short period of time" |
| Keywords: | Cre/loxP knockout mice lipid nanoparticles (LNPs); |
| Notes: | "PublisherMorita, Sumiyo Horii, Takuro Kimura, Mika Kobayashi, Ryosuke Tanaka, Hiroki Akita, Hidetaka Hatada, Izuho eng JP22ama121049/Japan Agency for Medical Research and Development/ Switzerland 2023/09/28 Int J Mol Sci. 2023 Sep 19; 24(18):14299. doi: 10.3390/ijms241814299" |
