| Title: | Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates |
| Author(s): | More MI; Finger LD; Stryker JL; Fuqua C; Eberhard A; Winans SC; |
| Address: | "Section of Microbiology, Cornell University, Ithaca, New York 14853, USA" |
| DOI: | 10.1126/science.272.5268.1655 |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "Many bacteria, including several pathogens of plants and humans, use a pheromone called an autoinducer to regulate gene expression in a cell density-dependent manner. Agrobacterium autoinducer [AAI, N-(3-oxo-octanoyl)-L-homoserine lactone] of A. tumefaciens is synthesized by the Tral protein, which is encoded by the tumor-inducing plasmid. Purified hexahistidinyl-Tral (H6-Tral) used S-adenosylmethionine to make the homoserine lactone moiety of AAI, but did not use related compounds. H6-Tral used 3-oxo-octanoyl-acyl carrier protein to make the 3-oxo-octanoyl moiety of AAI, but did not use 3-oxo-octanoyl-coenzyme A. These results demonstrate the enzymatic synthesis of an autoinducer through the use of purified substrates" |
| Keywords: | "3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/antagonists & inhibitors/metabolism Acyl Carrier Protein/metabolism Agrobacterium tumefaciens/*genetics/metabolism Base Sequence Cerulenin/pharmacology Chromatography, Gel Chromatography, High Pressure Liquid Clon;" |
| Notes: | "MedlineMore, M I Finger, L D Stryker, J L Fuqua, C Eberhard, A Winans, S C eng GM42893/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1996/06/14 Science. 1996 Jun 14; 272(5268):1655-8. doi: 10.1126/science.272.5268.1655" |
