| Title: | ANNEXIN1 mediates calcium-dependent systemic defense in Arabidopsis plants upon herbivory and wounding |
| Author(s): | Malabarba J; Meents AK; Reichelt M; Scholz SS; Peiter E; Rachowka J; Konopka-Postupolska D; Wilkins KA; Davies JM; Oelmuller R; Mithofer A; |
| Address: | "Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany. Postgraduate Program in Cell and Molecular Biology, Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre, RS, 90040-060, Brazil. Plant Physiology, Matthias-Schleiden-Institute for Genetics, Bioinformatics and Molecular Botany, Faculty of Biological Sciences, Friedrich-Schiller-University Jena, Jena, 07743, Germany. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Jena, 07745, Germany. Institute of Agricultural and Nutritional Sciences, Faculty of Natural Sciences III, Martin Luther University of Halle-Wittenberg, Halle (Saale), 06120, Germany. Plant Protein Phosphorylation Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, 02-106, Poland. Department of Plant Sciences, University of Cambridge, Cambridge, CB24 6DG, UK" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "Cellular calcium (Ca) transients are endogenous signals involved in local and systemic signaling and defense activation upon environmental stress, including wounding and herbivory. Still, not all Ca(2+) channels contributing to the signaling have been identified, nor are their modes of action fully known. Plant annexins are proteins capable of binding to anionic phospholipids and can exhibit Ca channel-like activity. Arabidopsis ANNEXIN1 (ANN1) is suggested to contribute to Ca transport. Here, we report that wounding and simulated-herbivory-induced cytosolic free Ca elevation was impaired in systemic leaves in ann1 loss-of-function plants. We provide evidence for a role of ANN1 in local and systemic defense of plants attacked by herbivorous Spodoptera littoralis larvae. Bioassays identified ANN1 as a positive defense regulator. Spodoptera littoralis feeding on ann1 gained significantly more weight than larvae feeding on wild-type, whereas those feeding on ANN1-overexpressing lines gained less weight. Herbivory and wounding both induced defense-related responses on treated leaves, such as jasmonate accumulation and defense gene expression. These responses remained local and were strongly reduced in systemic leaves in ann1 plants. Our results indicate that ANN1 plays an important role in activation of systemic rather than local defense in plants attacked by herbivorous insects" |
| Keywords: | "Animals *Arabidopsis/genetics/metabolism *Arabidopsis Proteins/genetics/metabolism Calcium/metabolism Cyclopentanes Gene Expression Regulation, Plant Herbivory Oxylipins Plant Leaves/metabolism Spodoptera calcium signaling jasmonates plant defense systemi;" |
| Notes: | "MedlineMalabarba, Jaiana Meents, Anja K Reichelt, Michael Scholz, Sandra S Peiter, Edgar Rachowka, Julia Konopka-Postupolska, Dorota Wilkins, Katie A Davies, Julia M Oelmuller, Ralf Mithofer, Axel eng BB/K009869/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2021/02/16 New Phytol. 2021 Jul; 231(1):243-254. doi: 10.1111/nph.17277. Epub 2021 Mar 19" |
