| Title: | "Chemical, Aroma and Pro-Health Characteristics of Kaffir Lime Juice-The Approach Using Optimized HS-SPME-GC-TOFMS, MP-OES, 3D-FL and Physiochemical Analysis" |
| Author(s): | Lubinska-Szczygel M; Polkowska Z; Rutkowska M; Gorinstein S; |
| Address: | "Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of Technology, 80-233 Gdansk, Poland. Institute for Drug Research, School of Pharmacy, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "The study aimed to provide the chemical, aroma and prohealth characteristics of the kaffir lime juice. A procedure using solid-phase microextraction with gas chromatography (SPME-GC-TOFMS) was optimized and validated for the determination of terpenes of kaffir lime. Main physicochemical parameters: pH, vitamin C, citric acid and degrees Brix were evaluated. Micro- and macro elements were determined using microwave plasma optic emission spectrometry (MP-OES). The binding of kaffir lime terpenes to human serum albumin (HSA) was investigated by fluorescence spectroscopy (3D-FL). beta-Pinene and Limonene were selected as the most abundant terpenes with the concentration of 1225 +/- 35 and 545 +/- 16 microg/g, respectively. The values of citric acid, vitamin C, degrees Brix and pH were 74.74 +/- 0.50 g/kg, 22.31 +/- 0.53 mg/100 mL, 10.35 +/- 0.70 and 2.406 +/- 0.086 for, respectively. Iron, with a concentration of 16.578 +/- 0.029 mg/kg, was the most abundant microelement. Among the macroelements, potassium (8121 +/- 52 mg/kg) was dominant. Kaffir lime binding to HSA was higher than beta-Pinene, which may indicate the therapeutic effect of the juice. Kaffir lime juice is a source of terpenes with good aromatic and bioactive properties. Fluorescence measurements confirmed its therapeutic effect. Kaffir lime juice is also a good source of citric acid with potential industrial application. The high content of minerals compared to other citruses increases its prohealth value" |
| Keywords: | Humans Odorants/analysis *Citrus/chemistry Gas Chromatography-Mass Spectrometry/methods Solid Phase Microextraction/methods Terpenes/analysis Spectrum Analysis Ascorbic Acid/analysis *Volatile Organic Compounds/analysis 3D fluorometry aroma properties gas; |
| Notes: | "MedlineLubinska-Szczygel, Martyna Polkowska, Zaneta Rutkowska, Malgorzata Gorinstein, Shela eng 2018/31/N/NZ9/03255/National Science Center/ Switzerland 2023/08/12 Int J Mol Sci. 2023 Aug 3; 24(15):12410. doi: 10.3390/ijms241512410" |
