| Title: | Determination of microbial volatile organic compounds from Staphylococcus pasteuri against Tuber borchii using solid-phase microextraction and gas chromatography/ion trap mass spectrometry |
| Author(s): | Barbieri E; Gioacchini AM; Zambonelli A; Bertini L; Stocchi V; |
| Address: | "Istituto di Chimica Biologica Giorgio Fornaini, Via Saffi 2, University of Urbino Carlo Bo, 61029 Urbino, Italy" |
| Journal Title: | Rapid Commun Mass Spectrom |
| ISSN/ISBN: | 0951-4198 (Print) 0951-4198 (Linking) |
| Abstract: | "The mycelium of Tuber borchii Vittad., a commercial truffle species, is used as a model system for in vitro ectomycorrhizal synthesis, infected seedling production and biotechnological applications. Our fungal cultures were accidentally contaminated with a Staphylococcus pasteuri strain, showing a strong antifungal activity against T. borchii mycelium. In order to identify the antifungal volatile agents produced by S. pasteuri, solid-phase microextraction (SPME) with gas chromatography and mass spectrometry (GC/MS) was used. Using this method 65 microbial volatile organic compounds (MVOCs), synthesized by this bacterium in either single or in fungal-bacterial dual culture, were identified. SPME combined with GC/MS may be a useful method for the determination of MVOCs involved in the antifungal activity. These results showed that bacteria with unusual biological activities could be a major problem during large-scale production of inoculum for truffle-infected seedling" |
| Keywords: | Fungi/*chemistry Gas Chromatography-Mass Spectrometry Organic Chemicals/*analysis/*chemistry Staphylococcus/*chemistry Volatilization; |
| Notes: | "MedlineBarbieri, E Gioacchini, A M Zambonelli, A Bertini, L Stocchi, V eng England 2005/11/01 Rapid Commun Mass Spectrom. 2005; 19(22):3411-5. doi: 10.1002/rcm.2209" |
