| Title: | Knockdown of CYP6CR2 and CYP6DE5 reduces tolerance to host plant allelochemicals in the Chinese white pine beetle Dendroctonus armandi |
| Author(s): | Liu B; Fu D; Ning H; Tang M; Chen H; |
| Address: | "College of Forestry, Northwest A&F University, Yangling 712100, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; College of Forestry, Northwest A&F University, Yangling 712100, China. Electronic address: tangm@nwsuaf.edu.cn. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; College of Forestry, Northwest A&F University, Yangling 712100, China. Electronic address: chenhui@nwsuaf.edu.cn" |
| DOI: | 10.1016/j.pestbp.2022.105180 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Bark beetles rely on detoxifying enzymes to resist the defensive terpenoids of the host tree. Insect cytochrome P450 (CYPs) plays a key role in the detoxification of pesticides and plant allelochemicals. CYP6 family is unique to Insecta, and its biochemical function is basically related to the metabolism of exogenous substances. In this study, we sequenced and characterized the full-length cDNAs of two CYP6 genes from Chinese white pine beetle, Dendroctonus armandi. Spatiotemporal expression profiling revealed that the expression of CYP6CR2 and CYP6DE5 was higher in larval and adult stages of D. armandi than that in other developmental stages, and that two genes predominantly expressed in brain, midgut, fat body, Malpighian tubules or hemolymph. The expression of CYP6CR2 and CYP6DE5 was significantly induced after feeding on the phloem of Pinus armandii and exposure to six stimuli [(+/-)- alpha -pinene, (-)-alpha-pinene, (-)-beta-pinene, (+)-3-carene, (+/-)-limonene and turpentine]. Importantly, silencing CYP6CR2 and CYP6DE5 separately could increase the sensitivity, led to a significant reduction of the activity of P450, resulting a significant increase in adult mortality after treatment with terpenoids. The comprehensive results of this study showed that in the process of host selection and colonization, the functions of CYPs were mainly to hydrolyze the chemical defense of the host and degrade odor molecules. These findings may help to develop new treatments to control this important pest" |
| Keywords: | Animals Bicyclic Monoterpenes China *Coleoptera/metabolism Cytochrome P-450 Enzyme System/metabolism Cytochrome P450 Family 6/metabolism Limonene Monoterpenes/metabolism/pharmacology *Pesticides/metabolism Pheromones/metabolism *Pinus Terpenes Turpentine/; |
| Notes: | "MedlineLiu, Bin Fu, Danyang Ning, Hang Tang, Ming Chen, Hui eng 2022/09/21 Pestic Biochem Physiol. 2022 Oct; 187:105180. doi: 10.1016/j.pestbp.2022.105180. Epub 2022 Jul 23" |
