| Title: | "Identification of volatile and nonvolatile compounds in Citri Reticulatae Pericarpium Viride using GC-MS, UPLC-Q-Exactive Orbitrap-MS, and HPLC-PDA" |
| Author(s): | Li J; Zou S; Yang W; Peng M; Chen B; Deng J; Wei M; Zheng G; |
| Address: | "Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital Guangzhou Medical University Guangzhou China. Guangdong Xinbaotang Biological Technology Co., Ltd Jiangmen China" |
| ISSN/ISBN: | 2048-7177 (Print) 2048-7177 (Electronic) 2048-7177 (Linking) |
| Abstract: | "Gas chromatograph-mass spectrometer (GC-MS), ultra-high-performance liquid chromatograph-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS), and high-performance liquid chromatography-photodiode array detection (HPLC-PDA) were used to qualitatively and quantitatively analyze the chemical component of Citri Reticulatae Pericarpium Viride 'Geqingpi' (GQP). First of all, the volatile components of GQP are identified by GC-MS. Totally 56 volatile components were determined, and gamma-Terpinene (33.39%) and D-Limonene (22.95%) were the main terpenes. Secondly, UHPLC-Q-Exactive Orbitrap-MS was used for identifying nonvolatile compositions and 42 compositions were identified totally, including 23 flavonoids, nine organic acids, three coumarins, two alkaloids compounds, and five other compounds, among which nine of the determined constituents were detected for the first time in GQP. Thirdly, the content of seven main constituents in GQP was quantitatively analyzed via HPLC-PDA, which were synephrine, hesperidin, limonin, nobiletin, HMF, tangeretin, and 5-HPMF. Further investigation for quantitative analysis of seven bioactive compounds suggested that the concentration of hesperidin in GQP approximately was 16.0% (160.78 +/- 0.95 mg.g(-1)), which was far higher than the standard for identification and quality control of CRPV in Chinese Pharmacopoeia (2020 edition) that 'the content of hesperidin shall not be less than 5.0%.' The phytochemicals of GQP were elucidated in this study, which might be supporting information for identification between GQP and Citri Reticulatae Pericarpium Viride 'Sihuaqingpi' (SHQP) and provided a scientific basis for the further active ingredient for pharmacological research and development prospects of GQP" |
| Keywords: | Citri Reticulatae Pericarpium Viride Gc-ms Hplc-pda UHPLC-Q-Exactive orbitrap-MS; |
| Notes: | "PubMed-not-MEDLINELi, Jingxuan Zou, Shiqi Yang, Wanling Peng, Mengdie Chen, Baizhong Deng, Jinji Wei, Minyan Zheng, Guodong eng 2023/03/14 Food Sci Nutr. 2023 Jan 4; 11(3):1415-1425. doi: 10.1002/fsn3.3181. eCollection 2023 Mar" |
