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Int J Mol Med

Title:		Bacterial-excreted small volatile molecule 2-aminoacetophenone induces oxidative stress and apoptosis in murine skeletal muscle
Author(s):		Bandyopadhaya A; Constantinou C; Psychogios N; Ueki R; Yasuhara S; Martyn JA; Wilhelmy J; Mindrinos M; Rahme LG; Tzika AA;
Address:		"Department of Surgery, Microbiology and Immunobiology, Harvard Medical School and Molecular Surgery Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Harvard Medical School, Boston, MA 02114, USA. NMR Surgical Laboratory, Center for Surgery, Innovation and Bioengineering, Department of Surgery, Massachusetts General and Shriners Burns Hospitals, Harvard Medical School, Boston, MA 02114, USA. Department of Anesthesiology and Critical Care, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA"
Journal Title:		Int J Mol Med
Year:		2016
Volume:		20160212
Issue:		4
Page Number:		867 - 878
DOI:		10.3892/ijmm.2016.2487
ISSN/ISBN:		1791-244X (Electronic) 1107-3756 (Print) 1107-3756 (Linking)
Abstract:		"Oxidative stress induces mitochondrial dysfunction and facilitates apoptosis, tissue damage or metabolic alterations following infection. We have previously discovered that the Pseudomonas aeruginosa (PA) quorum sensing (QS)-excreted small volatile molecule, 2-aminoacetophenone (2-AA), which is produced in infected human tissue, promotes bacterial phenotypes that favor chronic infection, while also dampening the pathogen?ª╡induced innate immune response, thus compromising muscle function and promoting host tolerance to infection. In this study, murine whole-genome expression data have demonstrated that 2-AA affects the expression of genes involved in reactive oxygen species (ROS) homeostasis, thus producing an oxidative stress signature in skeletal muscle. The results of the present study demonstrated that the expression levels of genes involved in apoptosis signaling pathways were upregulated in the skeletal muscle of 2-AA-treated mice. To confirm the results of our transcriptome analysis, we used a novel high-resolution magic-angle-spinning (HRMAS), proton (1H) nuclear magnetic resonance (NMR) method and observed increased levels of bisallylic methylene fatty acyl protons and vinyl protons, suggesting that 2-AA induces skeletal muscle cell apoptosis. This effect was corroborated by our results demonstrating the downregulation of mitochondrial membrane potential in vivo in response to 2-AA. The findings of the present study indicate that the bacterial infochemical, 2-AA, disrupts mitochondrial functions by inducing oxidative stress and apoptosis signaling and likely promotes skeletal muscle dysfunction, which may favor chronic/persistent infection"
Keywords:		"Acetophenones/*metabolism Animals *Apoptosis Gene Expression Regulation *Host-Pathogen Interactions Humans Male Membrane Potential, Mitochondrial Mice Mitochondria/genetics/metabolism/pathology Muscle, Skeletal/metabolism/*microbiology/pathology *Oxidativ;"
Notes:		"MedlineBandyopadhaya, Arunava Constantinou, Caterina Psychogios, Nikolaos Ueki, Ryusuke Yasuhara, Shingo Martyn, J A Jeevendra Wilhelmy, Julie Mindrinos, Michael Rahme, Laurence G Tzika, A Aria eng R21 AI105902/AI/NIAID NIH HHS/ R33 AI105902/AI/NIAID NIH HHS/ AI105902/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Greece 2016/03/05 Int J Mol Med. 2016 Apr; 37(4):867-78. doi: 10.3892/ijmm.2016.2487. Epub 2016 Feb 12"