« Previous Abstract"Evaluation of the physicochemical, metabolomic, and sensory characteristics of Chikso and Hanwoo beef during wet aging"    Next AbstractEffect of oxygen on volatile and sensory characteristics of Cabernet Sauvignon during secondary shelf life »

Proc Natl Acad Sci U S A

Title:		Natural genetic variation in the pheromone production of C. elegans
Author(s):		Lee D; Fox BW; Palomino DF; Panda O; Tenjo FJ; Koury EJ; Evans KS; Stevens L; Rodrigues PR; Kolodziej AR; Schroeder FC; Andersen EC;
Address:		"Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208. Department of Biology, Kyung Hee University, Seoul 02447, Republic of Korea. Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Republic of Korea. Boyce Thompson Institute, Cornell University, Ithaca, NY 14850. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14850. Tree of Life, Wellcome Sanger Institute, Cambridge CB10 1SA, United Kingdom"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2023
Volume:		20230620
Issue:		26
Page Number:		e2221150120 -
DOI:		10.1073/pnas.2221150120
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"From bacterial quorum sensing to human language, communication is essential for social interactions. Nematodes produce and sense pheromones to communicate among individuals and respond to environmental changes. These signals are encoded by different types and mixtures of ascarosides, whose modular structures further enhance the diversity of this nematode pheromone language. Interspecific and intraspecific differences in this ascaroside pheromone language have been described previously, but the genetic basis and molecular mechanisms underlying the variation remain largely unknown. Here, we analyzed natural variation in the production of 44 ascarosides across 95 wild Caenorhabditis elegans strains using high-performance liquid chromatography coupled to high-resolution mass spectrometry. We discovered wild strains defective in the production of specific subsets of ascarosides (e.g., the aggregation pheromone icas#9) or short- and medium-chain ascarosides, as well as inversely correlated patterns between the production of two major classes of ascarosides. We investigated genetic variants that are significantly associated with the natural differences in the composition of the pheromone bouquet, including rare genetic variants in key enzymes participating in ascaroside biosynthesis, such as the peroxisomal 3-ketoacyl-CoA thiolase, daf-22, and the carboxylesterase cest-3. Genome-wide association mappings revealed genomic loci harboring common variants that affect ascaroside profiles. Our study yields a valuable dataset for investigating the genetic mechanisms underlying the evolution of chemical communication"
Keywords:		Animals Humans *Caenorhabditis elegans/genetics Pheromones/chemistry Genome-Wide Association Study *Nematoda Genetic Variation C.elegans ascaroside fatty acid metabolism natural variation pheromone;
Notes:		"MedlineLee, Daehan Fox, Bennett W Palomino, Diana Fajardo Panda, Oishika Tenjo, Francisco J Koury, Emily J Evans, Kathryn S Stevens, Lewis Rodrigues, Pedro R Kolodziej, Aiden R Schroeder, Frank C Andersen, Erik C eng R35 GM131877/GM/NIGMS NIH HHS/ P40 OD010440/OD/NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2023/06/20 Proc Natl Acad Sci U S A. 2023 Jun 27; 120(26):e2221150120. doi: 10.1073/pnas.2221150120. Epub 2023 Jun 20"