| Title: | Growth media affect the volatilome and antimicrobial activity against Phytophthora infestans in four Lysobacter type strains |
| Author(s): | Lazazzara V; Perazzolli M; Pertot I; Biasioli F; Puopolo G; Cappellin L; |
| Address: | "Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy; Centre for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Strasse 20, 3430 Tulln an der Donau, Austria. Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy. Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy; Centre Agriculture Food Environment, University of Trento, Via Mach 1, 38010 San Michele all'Adige, Italy. Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy. Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy. Electronic address: gerardo.puopolo@fmach.it" |
| DOI: | 10.1016/j.micres.2017.04.015 |
| ISSN/ISBN: | 1618-0623 (Electronic) 0944-5013 (Linking) |
| Abstract: | "Bacterial volatile organic compounds (VOCs) play important ecological roles in soil microbial interactions. Lysobacter spp. are key determinants of soil suppressiveness against phytopathogens and the production of non-volatile antimicrobial metabolites has been extensively characterised. However, the chemical composition and antagonistic properties of the Lysobacter volatilome have been poorly investigated. In this work, VOC emission profiles of four Lysobacter type strains grown on a sugar-rich and a protein-rich medium were analysed using solid-phase microextraction gas chromatography-mass spectrometry and proton transfer reaction-time of flight-mass spectrometry. Lysobacter antibioticus, L. capsici, L. enzymogenes and L. gummosus type strains were recognised according to their volatilome assessed using both headspace mass spectrometry methods Moreover, the chemical profiles and functional properties of the Lysobacter volatilome differed according to the growth medium, and a protein-rich substrate maximised the toxic effect of the four Lysobacter type strains against Phytophthora infestans. Antagonistic (pyrazines, pyrrole and decanal) and non-antagonistic (delta-hexalactone and ethanol) VOCs against Ph. infestans or putative plant growth stimulator compounds (acetoin and indole) were mainly emitted by Lysobacter type strains grown on protein- and sugar-rich media respectively. Thus nutrient availability under soil conditions could affect the aggressiveness of Lysobacter spp. and possibly optimise interactions of these bacterial species with the other soil inhabitants" |
| Keywords: | Acetoin/metabolism Aldehydes/antagonists & inhibitors Anti-Infective Agents/*metabolism Biological Control Agents/antagonists & inhibitors Carbohydrate Metabolism Culture Media/*chemistry Ethanol/pharmacology Gas Chromatography-Mass Spectrometry/methods I; |
| Notes: | "MedlineLazazzara, Valentina Perazzolli, Michele Pertot, Ilaria Biasioli, Franco Puopolo, Gerardo Cappellin, Luca eng Germany 2017/06/13 Microbiol Res. 2017 Aug; 201:52-62. doi: 10.1016/j.micres.2017.04.015. Epub 2017 May 8" |
