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Biochem Biophys Res Commun

Title:		Ecm22 and Upc2 regulate yeast mating through control of expression of the mating genes PRM1 and PRM4
Author(s):		Hofken T;
Address:		"Division of Biosciences, Brunel University London, Uxbridge, UB8 3PH, UK. Electronic address: thomas.hoefken@brunel.ac.uk"
Journal Title:		Biochem Biophys Res Commun
Year:		2017
Volume:		20171003
Issue:		4
Page Number:		1485 - 1490
DOI:		10.1016/j.bbrc.2017.10.005
ISSN/ISBN:		1090-2104 (Electronic) 0006-291X (Linking)
Abstract:		"Budding yeast mating is an excellent model for receptor-activated cell differentiation. Here we identify the related transcription factors Ecm22 and Upc2 as novel regulators of mating. Cells lacking both ECM22 and UPC2 display strong mating defects whereas deletion of either gene has no effect. Ecm22 and Upc2 positively regulate basal expression of PRM1 and PRM4. These genes are strongly induced in response to mating pheromone, which is also largely dependent on ECM22 and UPC2. We further show that deletion of PRM4 like PRM1 results in markedly reduced mating efficiency. Expression of PRM1 but not of PRM4 is also regulated by Ste12, a key transcription factor for mating. STE12 deletion lowers basal PRM1 expression, whereas STE12 overexpression strongly increases PRM1 levels. This regulation of PRM1 transcription is mediated through three Ste12-binding sites in the PRM1 promoter. Simultaneous deletion of ECM22 and UPC2 as well as mutation of the three Ste12-binding sites in the PRM1 promoter completely abolishes basal and pheromone-induced PRM1 expression, indicating that Ste12 and Ecm22/Upc2 control PRM1 transcription through distinct pathways. In summary, we propose a novel mechanism for budding yeast mating. We suggest that Ecm22 and Upc2 regulate mating through the induction of the mating genes PRM1 and PRM4"
Keywords:		"Binding Sites/genetics Cation Transport Proteins/*genetics Gene Deletion Gene Expression Regulation, Fungal *Genes, Fungal Membrane Proteins/*genetics Pheromones/physiology Promoter Regions, Genetic Saccharomyces cerevisiae/cytology/*genetics/*physiology;"
Notes:		"MedlineHofken, Thomas eng 2017/10/08 Biochem Biophys Res Commun. 2017 Dec 2; 493(4):1485-1490. doi: 10.1016/j.bbrc.2017.10.005. Epub 2017 Oct 3"