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Insect Biochem Mol Biol

Title:		"Acyl-CoA Z9- and Z10-desaturase genes from a New Zealand leafroller moth species, Planotortrix octo"
Author(s):		Hao G; Liu W; O'Connor M; Roelofs W;
Address:		"Department of Entomology, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA"
Journal Title:		Insect Biochem Mol Biol
Year:		2002
Volume:		32
Issue:		9
Page Number:		961 - 966
DOI:		10.1016/s0965-1748(01)00176-x
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"Two cDNAs encoding acyl-CoA Z9-desaturase from the fat body and Z10-desaturase from the pheromone gland of the greenhead leafroller moth, Planotortrix octo, were obtained by RACE PCR. The Z9-desaturase (Pocto-Z9) cDNA spans 2291 nt with an ORF encoding a 352 amino-acid protein, which has 65% identity to Trichoplusia ni Delta 9 desaturase (Tni-Z9). The Z10-desaturase (Pocto-Z10) cDNA spans 2777 nt with an ORF encoding a protein with 356 amino acids. Pocto-Z10 shows lower identity to Pocto-Z9 and Tni-Z9 (48 and 46%, respectively) and relatively higher identity to the Delta 11 desaturases of T. ni and Helicoverpa zea (57 and 56%, respectively). The ORFs of these two P. octo cDNAs were constructed into an expression vector, YEpOLEX, that complemented the unsaturated fatty acid (UFA) auxotrophy of a desaturase-deficient ole1 strain of Saccharomyces cerevisiae. Expression of Pocto-Z9 produced a 5:2 ratio of Z9-16 and Z9-18 acids, with minor amounts (<4%) of Z9-14, Z9-15, and Z9-17 acids. Pocto-Z10 was successfully expressed in the YEpOLEX system when complemented with Z11-18:Me, and the major desaturase product proved to be Z10-16:Acid. The results confirm the regio- and stereo-selectivity of this unusual Delta 10 desaturase"
Keywords:		"Amino Acid Sequence Animals Base Sequence DNA, Complementary Fatty Acid Desaturases/*genetics/metabolism Female Molecular Sequence Data Moths/*enzymology/genetics New Zealand;"
Notes:		"MedlineHao, G Liu, W O'Connor, M Roelofs, W l eng Research Support, U.S. Gov't, Non-P.H.S. England 2002/09/06 Insect Biochem Mol Biol. 2002 Sep; 32(9):961-6. doi: 10.1016/s0965-1748(01)00176-x"