| Title: | Essential oils encapsulated in chitosan microparticles against Candida albicans biofilms |
| Author(s): | Garcia LGS; da Rocha MG; Lima LR; Cunha AP; de Oliveira JS; de Andrade ARC; Ricardo N; Pereira-Neto WA; Sidrim JJC; Rocha MFG; Vieira RS; Brilhante RSN; |
| Address: | "Department of Chemical Engineering, Federal University of Ceara, Fortaleza, CE, Brazil. School of Veterinary Medicine, Postgraduate Program in Veterinary Sciences, State University of Ceara, Fortaleza, CE, Brazil. Department of Organic and Inorganic Chemistry, Federal University of Ceara, Fortaleza, CE, Brazil. Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceara, Fortaleza, CE, Brazil. Department of Chemical Engineering, Federal University of Ceara, Fortaleza, CE, Brazil. Electronic address: rodrigogpsa@gmail.com. Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceara, Fortaleza, CE, Brazil. Electronic address: samiabrilhante@gmail.com" |
| DOI: | 10.1016/j.ijbiomac.2020.10.220 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate the essential oil (EO) release profile and the CMEOs' anti-Candida activity. The chitosan microparticles (CMs) loaded with lemongrass essential oil (LEO) and geranium essential oil (GEO) were produced by the spray-drying method and characterized regarding CMEO morphological and physicochemical parameters and EO encapsulation efficiency (EE) and release profile. The planktonic activity was quantified by broth microdilution, and the activity against biofilm was quantified by biomass formation measurement. The LEO and GEO compositions were analyzed by gas chromatography combined with mass spectrometry (GC/MS), finding the main components citral (83.17%) and citronellol (24.53%). The CMs and CMEOs showed regular distribution and spherical shape (1 to 15 mum), without any morphological and physical modifications after EO incorporation. EE% ranged from 12 to 39%. In vitro release tests demonstrated the EO release rates, after 144 h, were 33% and 55% in PBS and HCl media, respectively. The minimum inhibitory concentration (MIC) values for CMEOs were lower than for CMs and pure EOs (P < 0.05). The higher CMEO biofilm inhibition percentage demonstrates the efficiency of microparticles against Candida biofilm. These results indicate that CMEOs are promising compounds that have antibiofilm activity against C. albicans" |
| Keywords: | "Antifungal Agents/pharmacology Biofilms/*drug effects Candida albicans/*drug effects/*physiology Chitosan/*chemistry *Drug Compounding Gas Chromatography-Mass Spectrometry Geranium/chemistry Microbial Sensitivity Tests Oils, Volatile/*pharmacology Particl;" |
| Notes: | "MedlineGarcia, Lana Glerieide Silva da Rocha, Maria Gleiciane Lima, Laysa Rocha Cunha, Arcelina Pacheco de Oliveira, Jonathas Sales de Andrade, Ana Raquel Colares Ricardo, Nagila Maria Pontes Silva Pereira-Neto, Waldemiro Aquino Sidrim, Jose Julio Costa Rocha, Marcos Fabio Gadelha Vieira, Rodrigo Silveira Brilhante, Raimunda Samia Nogueira eng Netherlands 2020/11/03 Int J Biol Macromol. 2021 Jan 1; 166:621-632. doi: 10.1016/j.ijbiomac.2020.10.220. Epub 2020 Oct 31" |
