| Title: | Migration of phthalates on culture plates - an important challenge to consider for in vitro studies |
| Author(s): | Frohnert Hansen J; Boas M; Moller Brorson M; Frederiksen H; Hartoft-Nielsen ML; Krogh Rasmussen A; Main KM; Feldt-Rasmussen U; |
| Address: | "a Department of Medical Endocrinology , Rigshospitalet, University of Copenhagen , Denmark ; b Department of Growth and Reproduction , Rigshospitalet, University of Copenhagen , Denmark" |
| DOI: | 10.3109/00365513.2015.1110857 |
| ISSN/ISBN: | 1502-7686 (Electronic) 0036-5513 (Linking) |
| Abstract: | "Phthalates are endocrine disruptors of the reproductive system and suspected to influence many other organ and hormone systems. They are also semi-volatile organic compounds present in the gas phase in the environment. Their mode of action has been investigated in numerous in vitro studies. Multi-well culture plates are typically used to study phthalates in cell cultures. In a pilot study, we observed evidence of phthalate migration in 24-well culture plates. As this has not previously been described, we investigated the phenomenon in more detail. Primary human thyroid epithelial cell cultures (n = 8 cultures) were exposed to either di-ethyl phthalate (DEP), di-n-butyl phthalate (DnBP), mono-n-butyl phthalate (MnBP) or di-(2-ethylhexyl) phthalate (DEHP). Measurement of phthalate metabolites by mass spectrometry demonstrated that the short-branched DEP was able to migrate to adjacent wells when added to cell culture plates. DnBP also seemed to be able to migrate, unlike the long-branched DEHP or the monoester MnBP which did not seem to have this ability. High background levels of phthalate metabolites were also observed, which might compromise results from low dose phthalate studies. In conclusion, the migration of phthalates which is probably caused by their volatile properties might lead to false interpretation of study results" |
| Keywords: | "Cell Culture Techniques/instrumentation Cells, Cultured Endocrine Disruptors/analysis/*chemistry/pharmacology Epithelial Cells/drug effects/physiology Humans Phthalic Acids/analysis/*chemistry/pharmacology Pilot Projects Primary Cell Culture Thyroid Gland;" |
| Notes: | "MedlineFrohnert Hansen, Juliana Boas, Malene Moller Brorson, Marianne Frederiksen, Hanne Hartoft-Nielsen, Marie-Louise Krogh Rasmussen, Ase Main, Katharina M Feldt-Rasmussen, Ulla eng England 2016/01/13 Scand J Clin Lab Invest. 2016; 76(2):165-71. doi: 10.3109/00365513.2015.1110857. Epub 2016 Jan 12" |
