| Title: | In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease |
| Author(s): | Faria JM; Sena I; Vieira da Silva I; Ribeiro B; Barbosa P; Ascensao L; Bennett RN; Mota M; Figueiredo AC; |
| Address: | "Universidade de Lisboa, Faculdade de Ciencias de Lisboa, DBV, CESAM, Centro de Biotecnologia Vegetal, C2, Campo Grande, 1749-016, Lisbon, Portugal, jmfaria@fc.ul.pt" |
| DOI: | 10.1007/s00425-015-2257-9 |
| ISSN/ISBN: | 1432-2048 (Electronic) 0032-0935 (Linking) |
| Abstract: | "Co-cultures of Pinus pinaster with Bursaphelenchus xylophilus were established as a biotechnological tool to evaluate the effect of nematotoxics addition in a host/parasite culture system. The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), was detected for the first time in Europe in 1999 spreading throughout the pine forests in Portugal and recently in Spain. Plant in vitro cultures may be a useful experimental system to investigate the plant/nematode relationships in loco, thus avoiding the difficulties of field assays. In this study, Pinus pinaster in vitro cultures were established and compared to in vivo 1 year-old plantlets by analyzing shoot structure and volatiles production. In vitro co-cultures were established with the PWN and the effect of the phytoparasite on in vitro shoot structure, water content and volatiles production was evaluated. In vitro shoots showed similar structure and volatiles production to in vivo maritime pine plantlets. The first macroscopic symptoms of PWD were observed about 4 weeks after in vitro co-culture establishment. Nematode population in the culture medium increased and PWNs were detected in gaps of the callus tissue and in cavities developed from the degradation of cambial cells. In terms of volatiles main components, plantlets, P. pinaster cultures, and P. pinaster with B. xylophilus co-cultures were all beta- and alpha-pinene rich. Co-cultures may be an easy-to-handle biotechnological approach to study this pathology, envisioning the understanding of and finding ways to restrain this highly devastating nematode" |
| Keywords: | Animals Biotechnology/*methods Coculture Techniques/*methods Pinus/*growth & development/ultrastructure Plant Diseases/*parasitology Plant Shoots/growth & development/ultrastructure Tylenchida/*physiology Volatile Organic Compounds/analysis Water; |
| Notes: | "MedlineFaria, Jorge M S Sena, Ines Vieira da Silva, Ines Ribeiro, Bruno Barbosa, Pedro Ascensao, Lia Bennett, Richard N Mota, Manuel Figueiredo, A Cristina eng Research Support, Non-U.S. Gov't Germany 2015/02/14 Planta. 2015 Jun; 241(6):1325-36. doi: 10.1007/s00425-015-2257-9. Epub 2015 Feb 13" |
