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Plant Physiol Biochem

Title:		Monoterpene synthases contribute to the volatile production in tana (Zanthoxylum ailanthoides) through indigenous cultivation practices
Author(s):		Fan WL; Wen CH; Ma LT; Ho CL; Tung GS; Tien CC; Chu FH;
Address:		"School of Forestry and Resource Conservation, National Taiwan University, Taipei, 10617, Taiwan. Taiwan Forestry Research Institute, Taipei, 10066, Taiwan. Kalibuan, Xinyi Township, Nan-Tou, 55651, Taiwan. School of Forestry and Resource Conservation, National Taiwan University, Taipei, 10617, Taiwan. Electronic address: fhchu@ntu.edu.tw"
Journal Title:		Plant Physiol Biochem
Year:		2023
Volume:		20230816
Issue:
Page Number:		107969 -
DOI:		10.1016/j.plaphy.2023.107969
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"Tana (Zanthoxylum ailanthoides), a perennial deciduous species in the Rutaceae family, possesses leaves with a unique fragrance that indigenous peoples incorporate into their traditional cuisine. In Kalibuan, the cultivated tana trees were pruned repeatedly to maintain a shorter height, which led to the growth of new leaves that were spicier and pricklier. Tana leaves contain a range of volatile terpenoids, and the pungent aroma may arise from the presence of monoterpenoids. To gain insight into the biosynthetic pathway, five candidate monoterpene synthase genes were cloned and characterized using a purified recombinant protein assay. The main product of Za_mTPS1, Za_mTPS2, and Za_mTPS5 is sabinene, geraniol, and (E)-beta-ocimene, respectively. The main product of Za_mTPS3 and Za_mTPS4 is linalool. Real-time PCR analysis revealed that Za_mTPS1 and Za_mTPS5 are expressed at higher levels in prickly leaves of cultivated tana, suggesting that they may contribute to the distinctive aroma of this plant"
Keywords:		*Zanthoxylum/genetics *Apiaceae *Intramolecular Lyases Monoterpenes Ailanthus prickly ash Folklore plant Repruning Solid phase micro extraction Terpene synthase Volatile organic compounds;
Notes:		"MedlineFan, Wei-Lin Wen, Chi-Hsiang Ma, Li-Ting Ho, Chen-Lung Tung, Gene-Sheng Tien, Chin-Cheng Chu, Fang-Hua eng France 2023/08/20 Plant Physiol Biochem. 2023 Sep; 202:107969. doi: 10.1016/j.plaphy.2023.107969. Epub 2023 Aug 16"