| Title: | Genotoxicity of alkene epoxides in human peripheral blood mononuclear cells and HL60 leukaemia cells evaluated with the comet assay |
| Author(s): | Fabiani R; Rosignoli P; De Bartolomeo A; Fuccelli R; Morozzi G; |
| Address: | "Dipartimento di Specialita Medico-Chirurgiche e Sanita Pubblica, Sezione di Epidemiologia Molecolare e Igiene Ambientale, Universita di Perugia, via del Giochetto, 06126 Perugia, Italy. Electronic address: fabirob@unipg.it. Dipartimento di Specialita Medico-Chirurgiche e Sanita Pubblica, Sezione di Epidemiologia Molecolare e Igiene Ambientale, Universita di Perugia, via del Giochetto, 06126 Perugia, Italy" |
| DOI: | 10.1016/j.mrgentox.2012.01.004 |
| ISSN/ISBN: | 0027-5107 (Print) 0027-5107 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) exert their carcinogenic activity through the production of epoxide metabolites. Because of their high reactivity some epoxides are also produced in the chemical industry for the synthesis of other compounds. Therefore, human exposure to VOCs epoxides does occur and may be an important human health concern. In this study, the in vitro genotoxic potential of epoxides originating from 1,3-butadiene (3,4-epoxy-1-butene: EB; 1,2:3,4-diepoxybutane: DEB), isoprene (3,4-epoxy-2-methyl-1-butene: IO), styrene (styrene-7,8-oxide: SO), propylene (propylene oxide: PO) and 1-butene (1,2-epoxy-butane: BO) in human peripheral blood mononuclear cells (PBMCs) and promyelocytic leukaemia cells (HL60) was measured with the comet assay (single-cell gel electrophoresis, SCGE). The effect of inclusion of foetal calf serum (FCS, 5%) in the cell-culture medium and different durations of exposure (2h, 24h) were also investigated. All epoxides tested produced DNA damage in a concentration range that did not reduce cell viability. HL60 cells were more resistant than PBMCs to the DNA damage induced by the different epoxides. With the exception of IO, the treatment for 24h resulted in an increase of DNA damage. FCS slightly protected PBMCs from the genotoxic effects induced by IO and BO, whilst no such effect was noted for the other compounds. Overall, the dose-dependent effects that were seen allowed us to define a genotoxicity scale for the different epoxides as follows: SO>EB>DEB>IO>PO>BO, which is in partial agreement with the International Agency for Research on Cancer (IARC) classification of the carcinogenic hazards" |
| Keywords: | "Alkenes/toxicity Butadienes/toxicity *Comet Assay Epoxy Compounds/*toxicity HL-60 Cells/*drug effects Hemiterpenes/toxicity Humans Leukocytes, Mononuclear/*drug effects Mutagens/*toxicity Pentanes/toxicity;" |
| Notes: | "MedlineFabiani, Roberto Rosignoli, Patrizia De Bartolomeo, Angelo Fuccelli, Raffaela Morozzi, Guido eng Netherlands 2012/01/31 Mutat Res. 2012 Aug 30; 747(1):1-6. doi: 10.1016/j.mrgentox.2012.01.004. Epub 2012 Jan 21" |
