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Insect Sci

Title:		Expression pattern analysis of odorant-binding proteins in the pea aphid Acyrthosiphon pisum
Author(s):		De Biasio F; Riviello L; Bruno D; Grimaldi A; Congiu T; Sun YF; Falabella P;
Address:		"Dipartimento di Scienze, Universita della Basilicata, via dell' Ateneo Lucano 10, 85100, Potenza"
Journal Title:		Insect Sci
Year:		2015
Volume:		20140429
Issue:		2
Page Number:		220 - 234
DOI:		10.1111/1744-7917.12118
ISSN/ISBN:		1744-7917 (Electronic) 1672-9609 (Linking)
Abstract:		"Odorant-binding proteins (OBPs) are soluble proteins mediating chemoreception in insects. In previous research, we investigated the molecular mechanisms adopted by aphids to detect the alarm pheromone (E)-beta-farnesene and we found that the recognition of this and structurally related molecules is mediated by OBP3 and OBP7. Here, we show the differential expression patterns of 5 selected OBPs (OBP1, OBP3, OBP6, OBP7, OBP8) obtained performing quantitative RT-PCR and immunolocalization experiments in different body parts of adults and in the 5 developmental instars, including winged and unwinged morphs, of the pea aphid Acyrthosiphon pisum. The results provide an overall picture that allows us to speculate on the relationship between the differential expression of OBPs and their putative function. The expression of OBP3, OBP6, and OBP7 in the antennal sensilla suggests a chemosensory function for these proteins, whereas the constant expression level of OBP8 in all instars could suggest a conserved role. Moreover, OBP1 and OBP3 are also expressed in nonsensory organs. A light and scanning electron microscopy study of sensilla on different body parts of aphid, in particular antennae, legs, mouthparts, and cornicles-cauda, completes this research providing a guide to facilitate the mapping of OBP expression profiles"
Keywords:		"Animals Aphids/genetics/growth & development/*metabolism Arthropod Antennae/metabolism Gene Expression Profiling Insect Proteins/genetics/*metabolism Pheromones/metabolism Protein Binding Receptors, Odorant/genetics/*metabolism Sensilla/metabolism aphids;"
Notes:		"MedlineDe Biasio, Filomena Riviello, Lea Bruno, Daniele Grimaldi, Annalisa Congiu, Terenzio Sun, Yu Feng Falabella, Patrizia eng Research Support, Non-U.S. Gov't Australia 2014/03/05 Insect Sci. 2015 Apr; 22(2):220-34. doi: 10.1111/1744-7917.12118. Epub 2014 Apr 29"