« Previous AbstractExtreme aggression in male squid induced by a beta-MSP-like pheromone    Next AbstractMolecular Isomer Identification of Titan's Tholins Organic Aerosols by Photoelectron/Photoion Coincidence Spectroscopy Coupled to VUV Synchrotron Radiation »

J Exp Bot

Title:		The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis
Author(s):		Cumplido-Laso G; Medina-Puche L; Moyano E; Hoffmann T; Sinz Q; Ring L; Studart-Wittkowski C; Caballero JL; Schwab W; Munoz-Blanco J; Blanco-Portales R;
Address:		"Departamento de Bioquimica y Biologia Molecular. Edificio C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Cordoba, 14071 Cordoba, Spain"
Journal Title:		J Exp Bot
Year:		2012
Volume:		20120504
Issue:		11
Page Number:		4275 - 4290
DOI:		10.1093/jxb/ers120
ISSN/ISBN:		1460-2431 (Electronic) 0022-0957 (Linking)
Abstract:		"Short-chain esters contribute to the blend of volatiles that define the strawberry aroma. The last step in their biosynthesis involves an alcohol acyltransferase that catalyses the esterification of an acyl moiety of acyl-CoA with an alcohol. This study identified a novel strawberry alcohol acyltransferase gene (FaAAT2) whose expression pattern during fruit receptacle growth and ripening is in accordance with the production of esters throughout strawberry fruit ripening. The full-length FaAAT2 cDNA was cloned and expressed in Escherichia coli and its activity was analysed with acyl-CoA and alcohol substrates. The semi-purified FaAAT2 enzyme had activity with C1-C8 straight-chain alcohols and aromatic alcohols in the presence of acetyl-CoA. Cinnamyl alcohol was the most efficient acyl acceptor. When FaAAT2 expression was transiently downregulated in the fruit receptacle by agroinfiltration, the volatile ester production was significantly reduced in strawberry fruit. The results suggest that FaAAT2 plays a significant role in the production of esters that contribute to the final strawberry fruit flavour"
Keywords:		Acyltransferases/chemistry/genetics/*metabolism Alcohols/chemistry/metabolism Esters/chemistry/metabolism Fragaria/*enzymology/genetics/growth & development/metabolism Fruit/*enzymology/genetics/growth & development/metabolism Kinetics Molecular Sequence;
Notes:		"MedlineCumplido-Laso, Guadalupe Medina-Puche, Laura Moyano, Enriqueta Hoffmann, Thomas Sinz, Quirin Ring, Ludwig Studart-Wittkowski, Claudia Caballero, Jose Luis Schwab, Wilfried Munoz-Blanco, Juan Blanco-Portales, Rosario eng Research Support, Non-U.S. Gov't England 2012/05/09 J Exp Bot. 2012 Jun; 63(11):4275-90. doi: 10.1093/jxb/ers120. Epub 2012 May 4"