| Title: | "The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae" |
| Address: | "Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229, USA" |
| DOI: | 10.1128/EC.1.6.884-894.2002 |
| ISSN/ISBN: | 1535-9778 (Print) 1535-9786 (Electronic) 1535-9786 (Linking) |
| Abstract: | "A genetic selection in Saccharomyces cerevisiae for mutants that stimulate the mating pathway uncovered a mutant that had a hyperactive pheromone response pathway and also had hyperpolarized growth. Cloning and segregation analysis demonstrated that BUD14 was the affected gene. Disruption of BUD14 in wild-type cells caused mild stimulation of pheromone response pathway reporters, an increase in sensitivity to mating factor, and a hyperelongated shmoo morphology. The bud14 mutant also had hyperfilamentous growth. Consistent with a role in the control of cell polarity, a Bud14p-green fluorescent protein fusion was localized to sites of polarized growth in the cell. Bud14p shared morphogenetic functions with the Ste20p and Bni1p proteins as well as with the type 1 phosphatase Glc7p. The genetic interactions between BUD14 and GLC7 suggested a role for Glc7p in filamentous growth, and Glc7p was found to have a positive function in filamentous growth in yeast" |
| Keywords: | "Cell Division DNA Mutational Analysis Fluorescent Antibody Technique, Indirect Genotype Green Fluorescent Proteins Intracellular Signaling Peptides and Proteins Luminescent Proteins/metabolism MAP Kinase Kinase Kinases Mating Factor Microfilament Proteins;" |
| Notes: | "MedlineCullen, Paul J Sprague, George F Jr eng F32 GM019188/GM/NIGMS NIH HHS/ R01 GM030027/GM/NIGMS NIH HHS/ GM-30027/GM/NIGMS NIH HHS/ GM19188/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2002/12/13 Eukaryot Cell. 2002 Dec; 1(6):884-94. doi: 10.1128/EC.1.6.884-894.2002" |
