« Previous Abstract"Mesocosm trials of bioremediation of contaminated soil of a petroleum refinery: comparison of natural attenuation, biostimulation and bioaugmentation"    Next AbstractLoss of sustained Fus3p kinase activity and the G1 arrest response in cells expressing an inappropriate pheromone receptor »

Cell Tissue Res

Title:		Constancy and variability of identified glomeruli in antennal lobes: computational approach in Spodoptera littoralis
Author(s):		Couton L; Minoli S; Kieu K; Anton S; Rospars JP;
Address:		"INRA, UMR 1272 Physiologie de l'Insecte, Versailles, France"
Journal Title:		Cell Tissue Res
Year:		2009
Volume:		20090801
Issue:		3
Page Number:		491 - 511
DOI:		10.1007/s00441-009-0831-9
ISSN/ISBN:		1432-0878 (Electronic) 0302-766X (Linking)
Abstract:		"The primary olfactory centres share striking similarities across the animal kingdom. The most conspicuous is their subdivision into glomeruli, which are spherical neuropil masses in which synaptic contacts between sensory and central neurons occur. Glomeruli have both an anatomical identity (being invariant in location, size and shape) and a functional identity (each glomerulus receiving afferents from olfactory receptor neurons that express the same olfactory receptor). Identified glomeruli offer a favourable system for analysing quantitatively the constancy and variability of the neuronal circuits, an important issue for understanding their function, development and evolution. The noctuid moth Spodoptera littoralis with its well-studied pheromone communication system has become a model species for olfaction research. We analyse here its glomerular organisation based on ethyl-gallate-stained and synapsin-stained preparations. Although we have confirmed that the majority of glomeruli can be individually identified in various antennal lobes, we have recognised several types of biological variability. Some glomeruli are absent, possibly indicating the lack of the corresponding receptor neuron type or its misrouting during development. The antennal lobes vary in global shape and, consequently, the spatial location of the glomerular changes. Although they do not prevent glomerulus identification when quantitative analysis methods are used, these variations place limits on the straightforward identification of glomeruli in functional studies, e.g. calcium-imaging or single-cell staining, when using conventional three-dimensional maps of individual antennal lobes"
Keywords:		"Animals Computer Simulation Male Models, Biological Olfactory Receptor Neurons/cytology Spodoptera/*cytology Staining and Labeling;"
Notes:		"MedlineCouton, Louise Minoli, Sebastian Kieu, Kien Anton, Sylvia Rospars, Jean-Pierre eng Research Support, Non-U.S. Gov't Germany 2009/08/04 Cell Tissue Res. 2009 Sep; 337(3):491-511. doi: 10.1007/s00441-009-0831-9. Epub 2009 Aug 1"