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Molecules

Title:		Transcriptome and Metabolite Profiling Reveal Novel Insights into Volatile Heterosis in the Tea Plant (Camellia Sinensis)
Author(s):		Zheng Y; Wang P; Chen X; Sun Y; Yue C; Ye N;
Address:		"College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. 18094159524@163.com. College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. 2180311002@fafu.edu.cn. College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. 1180311002@fafu.edu.cn. College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. sunyun1125@126.com. College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. ynxtea@126.com. College of Horticulture, Key Laboratory of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China. yccyyx@163.com"
Journal Title:		Molecules
Year:		2019
Volume:		20190917
Issue:		18
Page Number:		-
DOI:		10.3390/molecules24183380
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Tea aroma is a key indicator for evaluating tea quality. Although notable success in tea aroma improvement has been achieved with heterosis breeding technology, the molecular basis underlying heterosis remains largely unexplored. Thus, the present report studies the tea plant volatile heterosis using a high-throughput next-generation RNA-seq strategy and gas chromatography-mass spectrometry. Phenotypically, we found higher terpenoid volatile and green leaf volatile contents by gas chromatography-mass spectrometry in the F1 hybrids than in their parental lines. Volatile heterosis was obvious in both F1 hybrids. At the molecular level, the comparative transcriptomics analysis revealed that approximately 41% (9027 of 21,995) of the genes showed non-additive expression, whereas only 7.83% (1723 of 21,995) showed additive expression. Among the non-additive genes, 42.1% showed high parental dominance and 17.6% showed over-dominance. Among different expression genes with high parental dominance and over-dominance expression patterns, KEGG and GO analyses found that plant hormone signal transduction, tea plant physiological process related pathways and most pathways associated with tea tree volatiles were enriched. In addition, we identified multiple genes (CsDXS, CsAATC2, CsSPLA2, etc.) and transcription factors (CsMYB1, CsbHLH79, CsWRKY40, etc.) that played important roles in tea volatile heterosis. Based on transcriptome and metabolite profiling, we conclude that non-additive action plays a major role in tea volatile heterosis. Genes and transcription factors involved in tea volatiles showing over-dominance expression patterns can be considered candidate genes and provide novel clues for breeding high-volatile tea varieties"
Keywords:		"Camellia sinensis/*genetics/*metabolism Computational Biology/methods Gene Expression Profiling Gene Expression Regulation, Plant Hybrid Vigor *Metabolome Metabolomics Plant Leaves/chemistry/genetics/metabolism *Transcriptome Volatile Organic Compounds/*m;"
Notes:		"MedlineZheng, Yucheng Wang, Pengjie Chen, Xuejin Sun, Yun Yue, Chuan Ye, Naixing eng Switzerland 2019/09/20 Molecules. 2019 Sep 17; 24(18):3380. doi: 10.3390/molecules24183380"