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Mol Plant Microbe Interact

Title:		Sensitivity and speed of induced defense of cabbage (Brassica oleracea L.): dynamics of BoLOX expression patterns during insect and pathogen attack
Author(s):		Zheng SJ; van Dijk JP; Bruinsma M; Dicke M;
Address:		"Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands"
Journal Title:		Mol Plant Microbe Interact
Year:		2007
Volume:		20
Issue:		11
Page Number:		1332 - 1345
DOI:		10.1094/MPMI-20-11-1332
ISSN/ISBN:		0894-0282 (Print) 0894-0282 (Linking)
Abstract:		"The lipoxygenase pathway is involved in the early steps of plant responses to herbivorous insects and phytopathogens. Induced defenses in the crucifer Brassica oleracea have been well documented. Here, we have cloned a LIPOXYGENASE (LOX) from B. oleracea (BoLOX). The sequence reveals that the BoLOX protein has a transit peptide for chloroplast targeting, which is characteristic for class 2 LOXs involved in jasmonic acid (JA) biosynthesis which takes place in the chloroplast. Phylogenetic analysis shows that BoLOX is closely related to B. napus BnLOX2fl and Arabidopsis thaliana AtLOX2, which mediates JA biosynthesis. BoLOX also shares functional characteristics with AtLOX2; BoLOX is inducible by wounding, JA treatment, and herbivores such as caterpillars (Pieris rapae, P. brassicae, and Mamestra brassicae), spider mites (Tetranychus urticae), locusts (Schistocerca gregaria), and a bacterial pathogen (Pseudomonas syringae pv. tomato). Of these, Pieris spp. caterpillars also induce AtLOX2 and JA biosynthesis in Arabidopsis. However, the aphid Myzus persicae did not induce BoLOX, which agrees with previous reports that this aphid induces neither AtLOX2 nor JA biosynthesis in Arabidopsis. Quantitative expression analysis of temporal, spatial, and density-dependent BoLOX transcript levels through real-time quantitative polymerase chain reaction demonstrated that BoLOX is maximally expressed after feeding by only two first-instar caterpillars for 24 h. Systemic expression was approximately 10-fold lower than local expression for herbivore-induced responses. The good correlation of BoLOX transcript levels with reports in the literature on induced defenses of B. oleracea is discussed"
Keywords:		"Amino Acid Sequence Animals Aphids/*physiology Brassica/drug effects/enzymology/*genetics/*physiology Cyclopentanes/pharmacology DNA, Complementary DNA, Plant Dose-Response Relationship, Drug Feeding Behavior *Gene Expression Regulation, Plant Lipoxygenas;"
Notes:		"MedlineZheng, Si-Jun van Dijk, Jeroen P Bruinsma, Maaike Dicke, Marcel eng Research Support, Non-U.S. Gov't 2007/11/06 Mol Plant Microbe Interact. 2007 Nov; 20(11):1332-45. doi: 10.1094/MPMI-20-11-1332"