| Title: | The SepOvotropin: a new ovarian peptide regulating oocyte transport in Sepia officinalis |
| Author(s): | Zatylny C; Gagnon J; Boucaud-Camou E; Henry J; |
| Address: | "Laboratoire de Biologie et Biotechnologies Marines, Universite de Caen, Caen cedex, 14032, France" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "In the cuttlefish Sepia officinalis, the successive steps of egg laying are controlled by multiple neuropeptides. Recent experiments led us to suppose that there was possible involvement of a second regulation pathway by the release of ovarian regulatory peptides in the genital tract. Using HPLC fractionation and an in vitro biological test, a C-terminal amidated peptide modulating the motility of the Sepia officinalis oviduct was isolated from an extract of vitellogenic ovarian follicles. The mass of this peptide as determined by MALDI-TOF (1501.8 Da) and analysis by Edman degradation led to the following sequence: Pro-Lys-Asp-Ser-Met-Leu-Leu-Leu-Gln-Val-Pro-Val-Tyr-amide. The peptide mapping performed by LC/MS revealed a distribution restricted to the follicles, the full grown oocytes and the eggs. This new peptide, called SepOvotropin, modulated contractions of the whole genital tract in physiological conditions from a threshold concentration between 10(-20) and 10(-19) M, demonstrating for the first time the occurrence of a specific peptidergic control of egg-laying in cephalopods" |
| Keywords: | "Amino Acid Sequence Animals Chromatography, High Pressure Liquid Female Molecular Weight Mollusca/*chemistry/drug effects/*physiology Muscle Contraction/drug effects Oocytes/chemistry/*drug effects/physiology Ovarian Follicle/*chemistry/drug effects/physi;" |
| Notes: | "MedlineZatylny, C Gagnon, J Boucaud-Camou, E Henry, J eng Research Support, Non-U.S. Gov't 2000/10/12 Biochem Biophys Res Commun. 2000 Oct 5; 276(3):1013-8. doi: 10.1006/bbrc.2000.3595" |
