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J Biotechnol

Title:		Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae
Author(s):		Yun EJ; Kwak S; Kim SR; Park YC; Jin YS; Kim KH;
Address:		"Department of Biotechnology, Graduate School, Korea University, Seoul 136-713, Republic of Korea. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. School of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea. Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 136-702, Republic of Korea. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: ysjin@illinois.edu. Department of Biotechnology, Graduate School, Korea University, Seoul 136-713, Republic of Korea. Electronic address: khekim@korea.ac.kr"
Journal Title:		J Biotechnol
Year:		2015
Volume:		20150528
Issue:
Page Number:		23 - 30
DOI:		10.1016/j.jbiotec.2015.05.017
ISSN/ISBN:		1873-4863 (Electronic) 0168-1656 (Linking)
Abstract:		"(S)-3-Hydroxybutyrate (S-3HB) can be used as a precursor for the synthesis of biodegradable polymers such as polyhydroxyalkanoate and stereo-specific fine chemicals such as antibiotics, pheromones, and drugs. For the production of S-3HB in yeast, the biosynthetic pathway of S-3HB from acetyl-CoA, consisting of the three enzymes, acetyl-CoA C-acetyltransferase (ACCT), acetoacetyl-CoA reductase (ACR), and 3-hydroxybutyryl-CoA thioesterase (HBT), was introduced into Saccharomyces cerevisiae. An engineered yeast strain overexpressing ERG10, hbd, and tesB genes not only exhibited enzyme activities of AACT, ACR, and HBT, but also produced S-3HB from ethanol. In order to increase the titer of S-3HB, a fed-batch fermentation based on pulse feeding of ethanol as a carbon source was performed, and a final S-3HB titer of 12.0g/L was achieved. This is the first report on the production of 3HB by engineered yeast, utilizing ethanol as the carbon source, suggesting that the industrially preferred S. cerevisiae can be a promising host for producing S-3HB"
Keywords:		3-Hydroxybutyric Acid/*biosynthesis Acetyl-CoA C-Acetyltransferase/genetics/metabolism Alcohol Oxidoreductases/genetics/metabolism Batch Cell Culture Techniques *Biosynthetic Pathways Ethanol/metabolism Fermentation Metabolic Engineering/*methods Saccharo;
Notes:		"MedlineYun, Eun Ju Kwak, Suryang Kim, Soo Rin Park, Yong-Cheol Jin, Yong-Su Kim, Kyoung Heon eng Research Support, Non-U.S. Gov't Netherlands 2015/06/01 J Biotechnol. 2015 Sep 10; 209:23-30. doi: 10.1016/j.jbiotec.2015.05.017. Epub 2015 May 28"