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BMC Genomics

Title:		Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments
Author(s):		Vita F; Giuntoli B; Bertolini E; Taiti C; Marone E; D'Ambrosio C; Trovato E; Sciarrone D; Zoccali M; Balestrini R; Scaloni A; Mondello L; Mancuso S; Alessio M; Alpi A;
Address:		"Dipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), University of Florence, Viale delle idee 30, 50019, Florence, Italy. federico.vita@unifi.it. A.R.E.A. Foundation, via Tavoleria 28, 56125, Pisa, Italy. federico.vita@unifi.it. Department of Biology, Universita di Pisa, via L. Ghini 13, 56126, Pisa, Italy. Institute of Life Sciences, Scuola Superiore Sant'Anna, Piazza Martiri della Liberta 33, 56127, Pisa, Italy. Present address: Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO, 63132, USA. Dipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), University of Florence, Viale delle idee 30, 50019, Florence, Italy. Faculty of Biosciences and Technologies for Agriculture Food and Environment, University of Teramo, Via R. Balzarini 1, 64100, Teramo, Italy. Proteomics and Mass Spectrometry Laboratory, I.S.P.A.A.M., National Research Council, 80147, Napoli, Italy. Chromaleont Srl, c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences Polo Annunziata, University of Messina, viale Annunziata, 98168, Messina, Italy. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Polo Annunziata, University of Messina, viale Annunziata, 98168, Messina, Italy. National Research Council of Italy, Institute for Sustainable Plant Protection (CNR-IPSP), Viale P.A. Mattioli 25, 10125, Torino, Italy. Division of Genetics and Cell Biology, IRCCS-Ospedale San Raffaele, Milan, Italy. A.R.E.A. Foundation, via Tavoleria 28, 56125, Pisa, Italy"
Journal Title:		BMC Genomics
Year:		2020
Volume:		20200129
Issue:		1
Page Number:		90 -
DOI:		10.1186/s12864-020-6522-3
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: Truffles are symbiotic fungi that develop underground in association with plant roots, forming ectomycorrhizae. They are primarily known for the organoleptic qualities of their hypogeous fruiting bodies. Primarily, Tuber magnatum Pico is a greatly appreciated truffle species mainly distributed in Italy and Balkans. Its price and features are mostly depending on its geographical origin. However, the genetic variation within T. magnatum has been only partially investigated as well as its adaptation to several environments. RESULTS: Here, we applied an integrated omic strategy to T. magnatum fruiting bodies collected during several seasons from three different areas located in the North, Center and South of Italy, with the aim to distinguish them according to molecular and biochemical traits and to verify the impact of several environments on these properties. With the proteomic approach based on two-dimensional electrophoresis (2-DE) followed by mass spectrometry, we were able to identify proteins specifically linked to the sample origin. We further associated the proteomic results to an RNA-seq profiling, which confirmed the possibility to differentiate samples according to their source and provided a basis for the detailed analysis of genes involved in sulfur metabolism. Finally, geographical specificities were associated with the set of volatile compounds produced by the fruiting bodies, as quantitatively and qualitatively determined through proton transfer reaction-mass spectrometry (PTR-MS) and gas-chromatography-mass spectrometry (GC-MS). In particular, a partial least squares-discriminant analysis (PLS-DA) model built from the latter data was able to return high confidence predictions of sample source. CONCLUSIONS: Results provide a characterization of white fruiting bodies by a wide range of different molecules, suggesting the role for specific compounds in the responses and adaptation to distinct environments"
Keywords:		"*Adaptation, Biological Computational Biology Electrophoresis, Gel, Two-Dimensional *Environment Gas Chromatography-Mass Spectrometry *Genomics/methods *Metabolomics/methods *Proteomics/methods Saccharomycetales/*genetics/*metabolism Transcriptome Volatil;"
Notes:		"MedlineVita, Federico Giuntoli, Beatrice Bertolini, Edoardo Taiti, Cosimo Marone, Elettra D'Ambrosio, Chiara Trovato, Emanuela Sciarrone, Danilo Zoccali, Mariosimone Balestrini, Raffaella Scaloni, Andrea Mondello, Luigi Mancuso, Stefano Alessio, Massimo Alpi, Amedeo eng VOLATOSCA/Regione Toscana/ England 2020/01/31 BMC Genomics. 2020 Jan 29; 21(1):90. doi: 10.1186/s12864-020-6522-3"