| Title: | Second-order input to the medial amygdala from olfactory sensory neurons expressing the transduction channel TRPM5 |
| Author(s): | Thompson JA; Salcedo E; Restrepo D; Finger TE; |
| Address: | "Rocky Mountain Taste and Smell Center, Department of Cell and Developmental Biology, Aurora, Colorado 80045, USA. john.a.thompson@ucdenver.edu" |
| ISSN/ISBN: | 1096-9861 (Electronic) 0021-9967 (Print) 0021-9967 (Linking) |
| Abstract: | "Recent anatomical tracing experiments in rodents have established that a subset of mitral cells in the main olfactory bulb (MOB) projects directly to the medial amygdala (MeA), traditionally considered a target of the accessory olfactory bulb. Neurons that project from the MOB to the MeA also show activation in response to conspecific (opposite sex) volatile urine exposure, establishing a direct role of the MOB in semiochemical processing. In addition, olfactory sensory neurons (OSNs) that express the transient receptor potential M5 (TRPM5) channel innervate a subset of glomeruli that respond to putative semiochemical stimuli. In this study, we examined whether the subset of glomeruli targeted by TRPM5-expressing OSNs is innervated by the population of mitral cells that projects to the MeA. We injected the retrograde tracer cholera toxin B (CTB) into the MeA of mice in which the TRPM5 promoter drives green fluorescent protein (GFP). We found overlapping clusters of CTB-labeled mitral cell dendritic branches (CTB(+) ) in TRPM5-GFP(+) glomeruli at significantly greater frequency than expected by chance. Despite the significant degree of colocalization, some amygdalopetal mitral cells extended dendrites to non-TRPM5-GFP glomeruli and vice versa, suggesting that, although significant overlapping glomerular innervation is observed between these two features, it is not absolute" |
| Keywords: | "Amygdala/*cytology/metabolism Animals Female Immunohistochemistry Male Mechanotransduction, Cellular/physiology Mice Mice, Inbred C57BL Microscopy, Confocal Olfactory Bulb/*cytology/metabolism Olfactory Pathways/cytology/metabolism Olfactory Receptor Neur;" |
| Notes: | "MedlineThompson, John A Salcedo, Ernesto Restrepo, Diego Finger, Thomas E eng P30 DC004657/DC/NIDCD NIH HHS/ R01 DC006070/DC/NIDCD NIH HHS/ DC006070/DC/NIDCD NIH HHS/ DC004657/DC/NIDCD NIH HHS/ Research Support, N.I.H., Extramural 2011/11/29 J Comp Neurol. 2012 Jun 1; 520(8):1819-30. doi: 10.1002/cne.23015" |
