| Title: | Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles |
| Author(s): | Stolterfoht H; Rinnofner C; Winkler M; Pichler H; |
| Address: | "Austrian Centre of Industrial Biotechnology , Petersgasse 14 , 8010 Graz , Austria. bisy e.U. , Wetzawinkel 20 , 8200 Hofstaetten , Austria. Institute of Molecular Biotechnology , TU Graz, NAWI Graz, BioTechMed Graz , Petersgasse 14 , 8010 Graz , Austria" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Green leaf volatiles (GLVs) are mainly C(6)- and in rare cases also C(9)-aldehydes, -alcohols, and -esters, which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focuses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids, such as linoleic and alpha-linolenic acids. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications, plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability, and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production" |
| Keywords: | Aldehyde-Lyases/*chemistry/genetics/metabolism Biocatalysis Cytochrome P-450 Enzyme System/*chemistry/genetics/metabolism Flavoring Agents/*chemistry/metabolism Lipoxygenases/*chemistry/genetics/metabolism Plant Leaves/chemistry/*enzymology/genetics Plant; |
| Notes: | "MedlineStolterfoht, Holly Rinnofner, Claudia Winkler, Margit Pichler, Harald eng Review 2019/10/09 J Agric Food Chem. 2019 Dec 11; 67(49):13367-13392. doi: 10.1021/acs.jafc.9b02690. Epub 2019 Nov 25" |
