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Sci Rep

Title:		Volatile metabolomic signature of human breast cancer cell lines
Author(s):		Silva CL; Perestrelo R; Silva P; Tomas H; Camara JS;
Address:		"CQM - Centro de Quimica da Madeira, Universidade da Madeira, Campus Universitario da Penteada, 9020-105 Funchal, Portugal. Departamento de Quimica, Faculdade de Ciencias Exatas e Engenharia da Universidade da Madeira, Universidade da Madeira, Campus Universitario da Penteada, 9020-105 Funchal, Portugal"
Journal Title:		Sci Rep
Year:		2017
Volume:		20170303
Issue:
Page Number:		43969 -
DOI:		10.1038/srep43969
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Breast cancer (BC) remains the most prevalent oncologic pathology in women, causing huge psychological, economic and social impacts on our society. Currently, the available diagnostic tools have limited sensitivity and specificity. Metabolome analysis has emerged as a powerful tool for obtaining information about the biological processes that occur in organisms, and is a useful platform for discovering new biomarkers or make disease diagnosis using different biofluids. Volatile organic compounds (VOCs) from the headspace of cultured BC cells and normal human mammary epithelial cells, were collected by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography combined with mass spectrometry (GC-MS), thus defining a volatile metabolomic signature. 2-Pentanone, 2-heptanone, 3-methyl-3-buten-1-ol, ethyl acetate, ethyl propanoate and 2-methyl butanoate were detected only in cultured BC cell lines. Multivariate statistical methods were used to verify the volatomic differences between BC cell lines and normal cells in order to find a set of specific VOCs that could be associated with BC, providing comprehensive insight into VOCs as potential cancer biomarkers. The establishment of the volatile fingerprint of BC cell lines presents a powerful approach to find endogenous VOCs that could be used to improve the BC diagnostic tools and explore the associated metabolomic pathways"
Keywords:		"Breast Neoplasms/*pathology Cell Line, Tumor Epithelial Cells/*pathology Gas Chromatography-Mass Spectrometry Humans *Metabolome Metabolomics Tumor Cells, Cultured Volatile Organic Compounds/*analysis;"
Notes:		"MedlineSilva, Catarina L Perestrelo, Rosa Silva, Pedro Tomas, Helena Camara, Jose S eng Research Support, Non-U.S. Gov't England 2017/03/04 Sci Rep. 2017 Mar 3; 7:43969. doi: 10.1038/srep43969"