| Title: | Soya bean Galpha proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant |
| Author(s): | Roy Choudhury S; Wang Y; Pandey S; |
| Address: | "*Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, U.S.A. daggerDepartment of Biology, Saint Louis University, 3507 Laclede Avenue, St. Louis, MO 63103, U.S.A" |
| ISSN/ISBN: | 1470-8728 (Electronic) 0264-6021 (Linking) |
| Abstract: | "Signalling pathways mediated by heterotrimeric G-proteins are common to all eukaryotes. Plants have a limited number of each of the G-protein subunits, with the most elaborate G-protein network discovered so far in soya bean (Glycine max, also known as soybean) which has four Galpha, four Gbeta and ten Ggamma proteins. Biochemical characterization of Galpha proteins from plants suggests significant variation in their properties compared with the well-characterized non-plant proteins. Furthermore, the four soya bean Galpha (GmGalpha) proteins exhibit distinct biochemical activities among themselves, but the extent to which such biochemical differences contribute to their in vivo function is also not known. We used the yeast gpa1 mutant which displays constitutive signalling and growth arrest in the pheromone-response pathway as an in vivo model to evaluate the effect of distinct biochemical activities of GmGalpha proteins. We showed that specific GmGalpha proteins can be activated during pheromone-dependent receptor-mediated signalling in yeast and they display different strengths towards complementation of yeast gpa1 phenotypes. We also identified amino acids that are responsible for differential complementation abilities of specific Galpha proteins. These data establish that specific plant Galpha proteins are functional in the receptor-mediated pheromone-response pathway in yeast and that the subtle biochemical differences in their activity are physiologically relevant" |
| Keywords: | "GTP-Binding Protein alpha Subunits/*chemistry/genetics/metabolism GTP-Binding Protein alpha Subunits, Gq-G11/*chemistry/genetics/metabolism Mutation/*genetics Phenotype Pheromones/pharmacology Saccharomyces cerevisiae/*chemistry/genetics Saccharomyces cer;" |
| Notes: | "MedlineRoy Choudhury, Swarup Wang, Yuqi Pandey, Sona eng Research Support, U.S. Gov't, Non-P.H.S. England 2014/04/04 Biochem J. 2014 Jul 1; 461(1):75-85. doi: 10.1042/BJ20131341" |
