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Toxicol Appl Pharmacol

Title:		Styrene induces an inflammatory response in human lung epithelial cells via oxidative stress and NF-kappaB activation
Author(s):		Roder-Stolinski C; Fischader G; Oostingh GJ; Feltens R; Kohse F; von Bergen M; Morbt N; Eder K; Duschl A; Lehmann I;
Address:		"UFZ-Helmholtz Centre for Environmental Research, Department of Environmental Immunology, Permoserstrasse 15, D-04318 Leipzig, Germany"
Journal Title:		Toxicol Appl Pharmacol
Year:		2008
Volume:		20080429
Issue:		2
Page Number:		241 - 247
DOI:		10.1016/j.taap.2008.04.010
ISSN/ISBN:		1096-0333 (Electronic) 0041-008X (Linking)
Abstract:		"Styrene is a volatile organic compound (VOC) that is widely used as a solvent in many industrial settings. Chronic exposure to styrene can result in irritation of the mucosa of the upper respiratory tract. Contact of styrene with epithelial cells stimulates the expression of a variety of inflammatory mediators, including the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). To characterise the underlying mechanisms of the induction of inflammatory signals by styrene, we investigated the influence of this compound on the induction of oxidative stress and the activation of the nuclear factor-kappa B (NF-kappaB) signalling pathway in human lung epithelial cells (A549). The results demonstrate that styrene-induced MCP-1 expression, as well as the expression of the oxidative stress marker glutathione S-transferase (GST), is associated with a concentration dependent pattern of NF-kappaB activity. An inhibitor of NF-kappaB, IKK-NBD, and the anti-inflammatory antioxidant N-acetylcysteine (NAC) were both effective in suppressing styrene-induced MCP-1 secretion. In addition, NAC was capable of inhibiting the upregulation of GST expression. Our findings suggest that the activation of the NF-kappaB signalling pathway by styrene is mediated via a redox-sensitive mechanism"
Keywords:		"Cell Line Chemokine CCL2/drug effects/metabolism Dose-Response Relationship, Drug Epithelial Cells/drug effects Gene Expression Regulation/drug effects Glutathione Transferase/drug effects/metabolism Humans Lung/*drug effects/metabolism NF-kappa B/*drug e;"
Notes:		"MedlineRoder-Stolinski, Carmen Fischader, Gundula Oostingh, Gertie Janneke Feltens, Ralph Kohse, Franziska von Bergen, Martin Morbt, Nora Eder, Klaus Duschl, Albert Lehmann, Irina eng 2008/06/17 Toxicol Appl Pharmacol. 2008 Sep 1; 231(2):241-7. doi: 10.1016/j.taap.2008.04.010. Epub 2008 Apr 29"