| Title: | Yeast alpha-mating factor receptor and G-protein-linked adenylyl cyclase inhibition requires RAS2 and GPA2 activities |
| Author(s): | Papasavvas S; Arkinstall S; Reid J; Payton M; |
| Address: | "Glaxo Institute for Molecular Biology, Chemin des Aulx, Geneva, Switzerland" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/s0006-291x(05)80035-x |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "Saccharomyces cerevisiae expresses two RAS gene products (RAS1 and RAS2) highly homologous to mammalian p21ras which mediate glucose-stimulated cyclic-AMP formation. Mating pheromone inhibits RAS-linked adenylyl cyclase activation and this is dependent upon the alpha-factor receptor (STE2) and its associated G-protein beta-subunit (STE4). We now show that this pheromone effect is independent of mating pathway signalling components 'downstream' of STE4 but displays an absolute requirement for an additional G-protein alpha-subunit encoded by GPA2. alpha-mating factor effects also involve a specific suppression of normal RAS2 activity as the constitutively activated mutant RAS2vall9 as well as wild type. RAS1 are insensitive to inhibition. Interaction between GPA2, STE4-STE18, RAS2 and adenylyl cyclase in yeast could give important insight into signalling pathways controlling normal and oncogenic p21ras activity in man" |
| Keywords: | "*Adenylyl Cyclase Inhibitors Cyclic AMP/metabolism Fungal Proteins/*metabolism GTP-Binding Proteins/*metabolism *Genes, ras Genetic Complementation Test Genotype Kinetics Macromolecular Substances Mating Factor Peptides/*pharmacology Phenotype Pheromones/;" |
| Notes: | "MedlinePapasavvas, S Arkinstall, S Reid, J Payton, M eng 1992/05/15 Biochem Biophys Res Commun. 1992 May 15; 184(3):1378-85. doi: 10.1016/s0006-291x(05)80035-x" |
