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Mater Sci Eng C Mater Biol Appl

Title:		Olfactory receptor-based polypeptide sensor for acetic acid VOC detection
Author(s):		Panigrahi S; Sankaran S; Mallik S; Gaddam B; Hanson AA;
Address:		"Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA; North Dakota State University, Fargo, ND, USA. Electrical and Computer Engineering Technology, Purdue University, West Lafayette, IN 47907, USA. Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58108, USA"
Journal Title:		Mater Sci Eng C Mater Biol Appl
Year:		2012
Volume:		20111112
Issue:		6
Page Number:		1307 - 1313
DOI:		10.1016/j.msec.2011.11.003
ISSN/ISBN:		1873-0191 (Electronic) 0928-4931 (Linking)
Abstract:		"Rapid detection of food-borne pathogens in packaged food products can prevent the spread of infectious diseases. This study investigates the application of novel sensing material that is sensitive to specific indicator volatile organic compound (VOC) related to Salmonella contamination in packaged meat. Specifically, the objective was to develop an olfactory receptor-based synthetic polypeptide sensor for the detecting acetic acid in low concentrations and at room temperature. Synthetic polypeptide was deposited on a quartz crystal microbalance (QCM) electrode and was evaluated for detecting acetic acid at 10-100 ppm. Developed sensor exhibited repeatable response to a particular concentration of acetic acid and displayed reproducibility among multiple sensors during acetic acid detection. Mean estimated lower detection limits of these sensors were about 1-3 ppm and linear calibration models showed linear relationships. Thus, the QCM sensors exhibited a great potential for detecting low concentrations of acetic acid at room temperature and can be used in the sensor array for packaged meat spoilage and contamination detection"
Keywords:		"Acetic Acid/*chemistry Electrodes Food Contamination/*analysis Indicators and Reagents/chemical synthesis Meat/analysis/microbiology Peptides/*chemistry Receptors, Odorant/*chemistry Reproducibility of Results Salmonella/chemistry Sensitivity and Specific;"
Notes:		"MedlinePanigrahi, Suranjan Sankaran, Sindhuja Mallik, Sanku Gaddam, Bhushan Hanson, Andrea A eng Research Support, U.S. Gov't, Non-P.H.S. Netherlands 2012/08/01 Mater Sci Eng C Mater Biol Appl. 2012 Aug 1; 32(6):1307-13. doi: 10.1016/j.msec.2011.11.003. Epub 2011 Nov 12"