| Title: | Unveiling Chemical Cues of Insect-Tree and Insect-Insect Interactions for the Eucalyptus Weevil and Its Egg Parasitoid by Multidimensional Gas Chromatographic Methods |
| Author(s): | Mendes D; Branco S; Paiva MR; Schutz S; Mateus EP; Gomes da Silva M; |
| Address: | "Associated Laboratory for Green Chemistry (LAQV) of the Network of Chemistry and Technology (REQUIMTE), Chemistry Department, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal. The Forest Research Centre (CEF), School of Agriculture University of Lisbon (ISA), Tapada da Ajuda, 1349-017 Lisbon, Portugal. Center for Environmental and Sustainability Research (CENSE), Department of Environmental Sciences and Engineering, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal. Department of Forest Zoology and Forest Conservation, Buesgen-Institute, Gottingen University, 37077 Gottingen, Germany" |
| DOI: | 10.3390/molecules27134042 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Multidimensional gas chromatography is, presently, an established and powerful analytical tool, due to higher resolving power than the classical 1D chromatographic approaches. Applied to multiple areas, it allows to isolate, detect and identify a larger number of compounds present in complex matrices, even in trace amounts. Research was conducted to determine which compounds, emitted by host plants of the eucalyptus weevil, Gonipterus platensis, might mediate host selection behavior. The identification of a pheromone blend of G. platensis is presented, revealing to be more attractive to weevils of both sexes, than the individual compounds. The volatile organic compounds (VOCs) were collected by headspace solid phase microextraction (HS-SPME), MonoTrap(TM) disks, and simultaneous distillation-extraction (SDE). Combining one dimensional (1D) and two-dimensional (2D) chromatographic systems-comprehensive and heart-cut two-dimensional gas chromatography (GCxGC and H/C-MD-GC, respectively) with mass spectrometry (MS) and electroantennographic (EAD) detection, enabled the selection and identification of pertinent semiochemicals which were detected by the insect antennal olfactory system. The behavioral effect of a selected blend of compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. An active blend, composed by cis and trans-verbenol, verbenene, myrtenol and trans-pinocarveol was achieved" |
| Keywords: | Animals Cues *Eucalyptus Female Gas Chromatography-Mass Spectrometry/methods Insecta Male Pheromones/chemistry Solid Phase Microextraction Trees *Volatile Organic Compounds/analysis *Weevils GCxGC Gonipterus platensis Md-gc chemical ecology pheromones; |
| Notes: | "MedlineMendes, Davide Branco, Sofia Paiva, Maria Rosa Schutz, Stefan Mateus, Eduardo P Gomes da Silva, Marco eng PDQS/BD/2022/Fundacao para a Ciencia e Tecnologia/ UID/QUI/50006/2020/Fundacao para a Ciencia e Tecnologia/ UIDB/05183/2020/Fundacao para a Ciencia e Tecnologia/ UID/AMB/04085/2020/Fundacao para a Ciencia e Tecnologia/ UID/AGR/00239/2019/Fundacao para a Ciencia e Tecnologia/ UIDB/00239/2020/Fundacao para a Ciencia e Tecnologia/ Project HOMED grant agreement no. 771271/H2020/ Switzerland 2022/07/10 Molecules. 2022 Jun 23; 27(13):4042. doi: 10.3390/molecules27134042" |
