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J Chromatogr A

Title:		Use of different sample temperatures in a single extraction procedure for the screening of the aroma profile of plant matrices by headspace solid-phase microextraction
Author(s):		Martendal E; de Souza Silveira CD; Nardini GS; Carasek E;
Address:		"Departamento de Quimica, Universidade Federal de Santa Catarina, Florianopolis, SC 88040-900, Brazil"
Journal Title:		J Chromatogr A
Year:		2011
Volume:		20110416
Issue:		24
Page Number:		3731 - 3736
DOI:		10.1016/j.chroma.2011.04.032
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"This study proposes a new approach to the optimization of the extraction of the volatile fraction of plant matrices using the headspace solid-phase microextraction (HS-SPME) technique. The optimization focused on the extraction time and temperature using a CAR/DVB/PDMS 50/30 mum SPME fiber and 100mg of a mixture of plants as the sample in a 15-mL vial. The extraction time (10-60 min) and temperature (5-60 degrees C) were optimized by means of a central composite design. The chromatogram was divided into four groups of peaks based on the elution temperature to provide a better understanding of the influence of the extraction parameters on the extraction efficiency considering compounds with different volatilities/polarities. In view of the different optimum extraction time and temperature conditions obtained for each group, a new approach based on the use of two extraction temperatures in the same procedure is proposed. The optimum conditions were achieved by extracting for 30 min with a sample temperature of 60 degrees C followed by a further 15 min at 5 degrees C. The proposed method was compared with the optimized conventional method based on a single extraction temperature (45 min of extraction at 50 degrees C) by submitting five samples to both procedures. The proposed method led to better results in all cases, considering as the response both peak area and the number of identified peaks. The newly proposed optimization approach provided an excellent alternative procedure to extract analytes with quite different volatilities in the same procedure"
Keywords:		"Chromatography, Gas Odorants/*analysis Plant Extracts/*chemistry Solid Phase Microextraction/*methods Temperature Time Factors Volatile Organic Compounds/*analysis;"
Notes:		"MedlineMartendal, Edmar de Souza Silveira, Cristine Durante Nardini, Giuliana Stael Carasek, Eduardo eng Comparative Study Research Support, Non-U.S. Gov't Netherlands 2011/05/14 J Chromatogr A. 2011 Jun 17; 1218(24):3731-6. doi: 10.1016/j.chroma.2011.04.032. Epub 2011 Apr 16"