| Title: | Hexokinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera |
| Author(s): | Chang Y; Zhang Y; Geng Z; Yao S; Zhao W; Yin X; An S; |
| Address: | "State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Acetyl-CoA, the precursor of sex pheromone biosynthesis in Helicoverpa armigera, is generated from glycolysis. As the first speed-limited enzyme in glycolysis, Hexokinase (HK) plays an important role in acetyl-CoA production. However, the function of HK in sex pheromone production remains unclear. This study employed H. armigera as material to explore the role of HK in sex pheromone production. Results demonstrated that the transcription profile of HaHK in female moth pheromone glands (PGs) was consistent with the release fluctuation of sex pheromone. Interference of HaHK prevented the increase of acetyl-CoA content induced by PBAN. Therefore, knockdown of HaHK in female PGs caused significant decreases in (Z)-11-hexadecenal (Z11-16:Ald) production, female capability to attract males, and mating rate. Furthermore, sugar feeding (5% sugar) increased the transcription and enzymatic activity of HK. Pheromone biosynthesis activating neuropeptide (PBAN) signal phospho-activated HaHK in PGs and Sf9 cells via protein kinase A (PKA), as shown by pharmacological inhibitor analysis. In general, our study confirmed that PBAN/cAMP/PKA signal activated HaHK, in turn promoted glycolysis to ensure the supply of acetyl-CoA, and finally facilitated sex pheromone biosynthesis and subsequent mating behavior" |
| Keywords: | Helicoverpa armigera Pban/pka Z11-16:Ald hexokinase mating behavior; |
| Notes: | "PubMed-not-MEDLINEChang, Yanpeng Zhang, Yunhui Geng, Zichen Yao, Shuangyan Zhao, Wenli Yin, Xinming An, Shiheng eng 31801339/National Natural Science Foundation of China/ 31772536/National Natural Science Foundation of China/ No.CARS-27/earmarked fund for China Agricultural Research system/ 202300410191/National Science fund of Henan Province for Distinguished Young scholar/ 21zx013/The Basic Research Project of the Key Scientific Research Projects of Universities in Henan Province/ Grant S2014-11-G06/Henan Agricultural Research System/ 212102110463/Science and Technology Project in Henan Province/ Switzerland 2021/10/24 Insects. 2021 Sep 30; 12(10):889. doi: 10.3390/insects12100889" |
