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Anal Biochem

Title:		Release of volatile organic compounds (VOCs) from colorectal cancer cell line LS174T
Author(s):		Liu M; Li Y; Wang G; Guo N; Liu D; Li D; Guo L; Zheng X; Yu K; Yu K; Wang C;
Address:		"Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China; Department of Anesthesiology, Xuanwu Hospital Capital Medical University, Beijing, China. Electronic address: liumiaomazui@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China; Department of Anesthesiology, The First Affiliated Hospital Sun Yat-sen University, Guangzhou, China. Electronic address: liyuhangmazui@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China; Department of Anesthesiology, Tianjin Medical University Cancer Hospital, Tianjin, China. Electronic address: lauruswang@yeah.net. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: guonanaicu2@163.com. Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: liudeshengmz@163.com. Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: dandanli119@163.com. Department of Anesthesiology, Xuanwu Hospital Capital Medical University, Beijing, China. Electronic address: 57077184@qq.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: zhengxiaoya555@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: yukaili1543@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China; Department of Critical Care Medicine, The first Affiliated Hospital of Harbin Medical University, China. Electronic address: drkaijiang@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: changsongwangicu@163.com"
Journal Title:		Anal Biochem
Year:		2019
Volume:		20190618
Issue:
Page Number:		113340 -
DOI:		10.1016/j.ab.2019.06.011
ISSN/ISBN:		1096-0309 (Electronic) 0003-2697 (Linking)
Abstract:		"Colorectal cancer (CRC) is the third most common cancer worldwide. To date, no non-invasive and specific biomarkers have been identified for the diagnosis of CRC. The analysis of volatile organic compounds (VOCs) is attracting increasing attention and provides the possibility of a non-invasive diagnosis. Solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) have been used to analyze the VOCs released from the headspace gas of LS174T (Dukes' type B colorectal adenocarcinoma) cells, arsenic trioxide (ATO)-treated LS174T cells and the blood from tumor-bearing mice. The data were processed using principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA), which showed that the levels of decanal, 2,4-dimethyl- heptane, and twelve other metabolites were significantly greater in the headspace gas of the LS174T cells and blood of tumor-bearing mice. Additionally, in vivo experiments indicated that formic acid, ethenyl ester and p-trimethylsilyloxyphenyl-(trimethylsilyloxy)trimethylsilylacrylate were consumed during tumor growth. In conclusion, VOCs such as 1-methoxy-hexane and 2,4-dimethyl-heptane could be useful diagnostic markers for CRC. Further research should focus on the potential metabolic pathways associated with these profiles"
Keywords:		"Animals Arsenic Trioxide/pharmacology Cell Line, Tumor Colorectal Neoplasms/drug therapy/*metabolism/pathology Female Gas Chromatography-Mass Spectrometry Humans Mice, Inbred BALB C Mice, Nude Volatile Organic Compounds/analysis/*metabolism Colorectal can;"
Notes:		"MedlineLiu, Miao Li, Yuhang Wang, Guiyue Guo, Nana Liu, Desheng Li, Dandan Guo, Lei Zheng, Xiaoya Yu, Kaili Yu, Kaijiang Wang, Changsong eng Research Support, Non-U.S. Gov't 2019/06/22 Anal Biochem. 2019 Sep 15; 581:113340. doi: 10.1016/j.ab.2019.06.011. Epub 2019 Jun 18"