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J Vis Exp


Title:Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals
Author(s):Liu F; Liu N;
Address:"Department of Entomology and Plant Pathology, Auburn University. Department of Entomology and Plant Pathology, Auburn University; liunann@auburn.edu"
Journal Title:J Vis Exp
Year:2016
Volume:20160118
Issue:107
Page Number:e53337 -
DOI: 10.3791/53337
ISSN/ISBN:1940-087X (Electronic) 1940-087X (Linking)
Abstract:"The insect olfactory system plays an important role in detecting semiochemicals in the environment. In particular, the antennal sensilla which house single or multiple neurons inside, are considered to make the major contribution in responding to the chemical stimuli. By directly recording action potential in the olfactory sensillum after exposure to stimuli, single sensillum recording (SSR) technique provides a powerful approach for investigating the neural responses of insects to chemical stimuli. For the bed bug, which is a notorious human parasite, multiple types of olfactory sensillum have been characterized. In this study, we demonstrated neural responses of bed bug olfactory sensilla to two chemical stimuli and the dose-dependent responses to one of them using the SSR method. This approach enables researchers to conduct early screening for individual chemical stimuli on the bed bug olfactory sensilla, which would provide valuable information for the development of new bed bug attractants or repellents and benefits the bed bug control efforts"
Keywords:Animals Bedbugs/cytology/*physiology Neurons/*physiology Pheromones/*chemistry Sensilla/*physiology Smell/*physiology;
Notes:"MedlineLiu, Feng Liu, Nannan eng Research Support, Non-U.S. Gov't Video-Audio Media 2016/02/11 J Vis Exp. 2016 Jan 18; (107):e53337. doi: 10.3791/53337"

 
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