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J Agric Food Chem

Title:		Transcriptome and Metabolite Conjoint Analysis Reveals that Exogenous Methyl Jasmonate Regulates Monoterpene Synthesis in Grape Berry Skin
Author(s):		Li W; Li W; Yang S; Ma Z; Zhou Q; Mao J; Han S; Chen B;
Address:		"College of Horticulture, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China. Gansu Key Laboratory of Viticulture and Enology, Lanzhou 730070, Gansu Province, China"
Journal Title:		J Agric Food Chem
Year:		2020
Volume:		20200427
Issue:		18
Page Number:		5270 - 5281
DOI:		10.1021/acs.jafc.0c00476
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Monoterpene is one of the important sources of varietal aroma, which provides a strong floral and fruity aroma in wines. Methyl jasmonate (MeJA) affects plant secondary metabolism. However, the regulatory mechanisms of monoterpene biosynthesis after MeJA application on grapes are not illuminated. In the present study, 10 mM MeJA was used as treatments in Italian Riesling grape at the preveraison stage in different ways, including grape cluster soaking, foliar spraying, and whole vine spraying, designated as T1, T2, and T3, respectively, while a blank group was used as the control (CK). HS-SPME/GC-MS and transcriptome sequencing analysis were performed to investigate the effect of exogenous MeJA on monoterpene synthesis in grape berry skin. The results of GC-MS showed that the application of MeJA induced the accumulation of volatile monoterpenes in grape berry skin, especially linalool, alpha-terpineol, and oxides. In addition, transcriptome analysis showed that differentially expressed genes were increased from T2 to T3 to T1 compared with CK, and significantly enriched in JA and monoterpene synthesis pathways. T1 application significantly upregulated the mRNA expression levels of LOX2S, AOS, OPR, and JMT involved in the JA biosynthesis pathway, as well as DXS, HMGCR, TPS14, and alpha-terpineol synthesis genes involved in the monoterpene synthesis pathway compared with T2, T3, and CK. Thus, grape cluster soaking treatment with MeJA could greatly activate volatile monoterpene synthesis. The results will deeply increase our understanding of the monoterpene biosynthesis of grape berry skin in response to MeJA"
Keywords:		"Acetates/*pharmacology Biosynthetic Pathways Cyclopentanes/*pharmacology Fruit/*drug effects/genetics/metabolism Gene Expression Profiling Gene Expression Regulation, Plant/*drug effects Monoterpenes/*metabolism Oxylipins/*pharmacology Plant Growth Regula;"
Notes:		"MedlineLi, Wei Li, Wenfang Yang, Shijin Ma, Zonghuan Zhou, Qi Mao, Juan Han, Shunyu Chen, Baihong eng 2020/04/28 J Agric Food Chem. 2020 May 6; 68(18):5270-5281. doi: 10.1021/acs.jafc.0c00476. Epub 2020 Apr 27"