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Curr Genet


Title:"Identification and linkage mapping of the genes for the putative homeodomain protein (hox1) and the putative pheromone receptor protein homologue (rcb1) in a bipolar basidiomycete, Pholiota nameko"
Author(s):Aimi T; Yoshida R; Ishikawa M; Bao D; Kitamoto Y;
Address:"Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori, 680-8553 Japan. taimi@muses.tottori-u.ac.jp"
Journal Title:Curr Genet
Year:2005
Volume:20051012
Issue:3
Page Number:184 - 194
DOI: 10.1007/s00294-005-0012-7
ISSN/ISBN:0172-8083 (Print) 0172-8083 (Linking)
Abstract:"In the current studies, we sequenced and characterized the gene for the homeodomain protein (hox1) in a bipolar mushroom, Pholiota nameko, which is a putative homologue of A mating type genes in the tetrapolar basidiomycete, Coprinopsis cinerea. We also sequenced and characterized the gene for the pheromone receptor (rcb1) in P. nameko, which is a putative homologue of the B mating type genes in C. cinerea. Restriction fragment length polymorphism (RFLP) and linkage analyses indicated that the both genes are present as a single locus on the different chromosome. Moreover, in P. nameko, the hox1 gene was mapped to the A mating type locus in linkage group I. However, rcb1 was not linked to the A mating type locus and was mapped to the other linkage group. These results strongly suggest that hox1 regulates with incompatibility in the bipolar mushroom, and that rcb1 may not affect the mating function in P. nameko. This is the first report regarding the structure of the mating type genes in bipolar mushrooms"
Keywords:"Amino Acid Sequence Base Sequence Basidiomycota/*genetics Blotting, Southern Chromosome Mapping Cloning, Molecular DNA, Fungal Fungal Proteins/*genetics Genes, Fungal Homeodomain Proteins/*genetics Molecular Sequence Data Nucleic Acid Amplification Techni;"
Notes:"MedlineAimi, Tadanori Yoshida, Rie Ishikawa, Mariko Bao, Dapeng Kitamoto, Yutaka eng Review 2005/08/13 Curr Genet. 2005 Sep; 48(3):184-94. doi: 10.1007/s00294-005-0012-7. Epub 2005 Oct 12"

 
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