| Title: | "Screening of Satureja subspicata Vis. Honey by HPLC-DAD, GC-FID/MS and UV/VIS: Prephenate Derivatives as Biomarkers" |
| Author(s): | Jerkovic I; Kranjac M; Marijanovic Z; Zekic M; Radonic A; Tuberoso CI; |
| Address: | "Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, Split 21000, Croatia. igor@ktf-split.hr. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, Split 21000, Croatia. mkranjac@ktf-split.hr. Department of Food Technology, Marko Marulic Polytechnic in Knin, Petra Kresimira IV 30, Knin 22300, Croatia. zmarijanovic@veleknin.hr. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, Split 21000, Croatia. zekic@ktf-split.hr. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, Split 21000, Croatia. radonic@ktf-split.hr. Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, Cagliari 09124, Italy. tuberoso@unica.it" |
| DOI: | 10.3390/molecules21030377 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The samples of Satureja subspicata Vis. honey were confirmed to be unifloral by melissopalynological analysis with the characteristic pollen share from 36% to 71%. Bioprospecting of the samples was performed by HPLC-DAD, GC-FID/MS, and UV/VIS. Prephenate derivatives were shown to be dominant by the HPLC-DAD analysis, particularly phenylalanine (167.8 mg/kg) and methyl syringate (MSYR, 114.1 mg/kg), followed by tyrosine and benzoic acid. Higher amounts of MSYR (3-4 times) can be pointed out for distinguishing S. subspicata Vis. honey from other Satureja spp. honey types. GC-FID/MS analysis of ultrasonic solvent extracts of the samples revealed MSYR (46.68%, solvent pentane/Et2O 1:2 (v/v); 52.98%, solvent CH2Cl2) and minor abundance of other volatile prephenate derivatives, as well as higher aliphatic compounds characteristic of the comb environment. Two combined extracts (according to the solvents) of all samples were evaluated for their antioxidant properties by FRAP and DPPH assay; the combined extracts demonstrated higher activity (at lower concentrations) in comparison with the average honey sample. UV/VIS analysis of the samples was applied for determination of CIE Lab colour coordinates, total phenolics (425.38 mg GAE/kg), and antioxidant properties (4.26 mmol Fe(2+)/kg (FRAP assay) and 0.8 mmol TEAC/kg (DDPH assay))" |
| Keywords: | "Antioxidants/chemistry Biomarkers/chemistry *Chromatography, High Pressure Liquid Cyclohexanecarboxylic Acids/*chemistry/isolation & purification Cyclohexenes/*chemistry/isolation & purification *Gas Chromatography-Mass Spectrometry Honey/*analysis Phenol;" |
| Notes: | "MedlineJerkovic, Igor Kranjac, Marina Marijanovic, Zvonimir Zekic, Marina Radonic, Ani Tuberoso, Carlo Ignazio Giovanni eng Research Support, Non-U.S. Gov't Switzerland 2016/03/24 Molecules. 2016 Mar 21; 21(3):377. doi: 10.3390/molecules21030377" |
