| Title: | Statistical Evaluation of Total Expiratory Breath Samples Collected throughout a Year: Reproducibility and Applicability toward Olfactory Sensor-Based Breath Diagnostics |
| Author(s): | Inada K; Kojima H; Cho-Isoda Y; Tamura R; Imamura G; Minami K; Nemoto T; Yoshikawa G; |
| Address: | "Department of Medical Oncology, Ibaraki Prefectural Central Hospital, Kasama 309-1793, Ibaraki, Japan. Ibaraki Clinical Education and Training Center, University of Tsukuba Hospital, Kasama 309-1793, Ibaraki, Japan. World Premier International (WPI) Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), Tsukuba 305-0044, Ibaraki, Japan. Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8568, Japan. Research and Services Division of Materials Data and Integrated System (MaDIS), National Institute for Materials Science (NIMS), Tsukuba 305-0044, Japan. International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS), Tsukuba 305-0044, Ibaraki, Japan. Center for Functional Sensor & Actuator (CFSN), Research Center for Functional Materials, National Institute for Materials Science (NIMS), Tsukuba 305-0044, Japan. Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, Tsukuba 305-8571, Ibaraki, Japan" |
| ISSN/ISBN: | 1424-8220 (Electronic) 1424-8220 (Linking) |
| Abstract: | "The endogenous volatile organic compounds (VOCs) in exhaled breath can be promising biomarkers for various diseases including cancers. An olfactory sensor has a possibility for extracting a specific feature from collective variations of the related VOCs with a certain health condition. For this approach, it is important to establish a feasible protocol for sampling exhaled breath in practical conditions to provide reproducible signal features. Here we report a robust protocol for the breath analysis, focusing on total expiratory breath measured by a Membrane-type Surface stress Sensor (MSS), which possesses practical characteristics for artificial olfactory systems. To assess its reproducibility, 83 exhaled breath samples were collected from one subject throughout more than a year. It has been confirmed that the reduction of humidity effects on the sensing signals either by controlling the humidity of purging room air or by normalizing the signal intensities leads to reasonable reproducibility verified by statistical analyses. We have also demonstrated the applicability of the protocol for detecting a target material by discriminating exhaled breaths collected from different subjects with pre- and post-alcohol ingestion on different occasions. This simple yet reproducible protocol based on the total expiratory breath measured by the MSS olfactory sensors will contribute to exploring the possibilities of clinical applications of breath diagnostics" |
| Keywords: | Biomarkers *Breath Tests Exhalation Humans Reproducibility of Results *Volatile Organic Compounds Membrane-type Surface stress Sensor (MSS) artificial olfactory sensor breath analysis humidity nanomechanical sensor reproducibility; |
| Notes: | "MedlineInada, Katsushige Kojima, Hiroshi Cho-Isoda, Yukiko Tamura, Ryo Imamura, Gaku Minami, Kosuke Nemoto, Takahiro Yoshikawa, Genki eng 18K07318/Japan Society for the Promotion of Science/ 18H04168/Japan Society for the Promotion of Science/ 20K20554/Japan Society for the Promotion of Science/ JPMJCR1665/Japan Science and Technology Agency/ Switzerland 2021/07/25 Sensors (Basel). 2021 Jul 11; 21(14):4742. doi: 10.3390/s21144742" |
