« Previous AbstractTranscriptome and Volatilome Analysis During Growth of Brochothrix thermosphacta in Food: Role of Food Substrate and Strain Specificity for the Expression of Spoilage Functions    Next AbstractPheromonal attraction of Schistosoma mansoni females toward males in the absence of worm-tactile behavior »

Mol Cell Biol

Title:		Schizosaccharomyces pombe sxa1+ and sxa2+ encode putative proteases involved in the mating response
Author(s):		Imai Y; Yamamoto M;
Address:		"Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan"
Journal Title:		Mol Cell Biol
Year:		1992
Volume:		12
Issue:		4
Page Number:		1827 - 1834
DOI:		10.1128/mcb.12.4.1827-1834.1992
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The Schizosaccharomyces pombe sxa1 and sxa2 mutants showed an exaggerated response to mating pheromones, producing excessively long conjugation tubes and exhibiting mating deficiency. This phenotype was similar to phenotypes of cells bearing an activated allele of ras1, such as ras1Val-17 or ras1Leu-66, and phenotypes of cells defective in gap1. However, genetic evidence suggested that the sxa1 and sxa2 gene products are not directly involved in the Ras1 pathway. The gene products of sxa1 and sxa2, as deduced from their nucleotide sequences, were homologous to aspartyl proteases and serine carboxypeptidases, respectively. The sxa1 gene function was required for efficient mating only in h+ cells, although even disruption of sxa1 did not completely abolish the mating ability. Conversely, the sxa2 gene function was required only in h- cells. Wild-type cells produced a diffusible substance, which may be the sxa2 gene product itself, that could confer fertility to sxa2 mutant cells placed at a distance. These observations are consistent with the possibility that the sxa gene products are involved in degradation or processing of the mating pheromones and that their loss cause a persistent response to the pheromones"
Keywords:		"Amino Acid Sequence Aspartic Acid Endopeptidases/genetics Base Sequence Cell Communication Cloning, Molecular Conjugation, Genetic/*genetics DNA Mutational Analysis Endopeptidases/*genetics Genes, ras/genetics Molecular Sequence Data Mutation Phenotype Re;"
Notes:		"MedlineImai, Y Yamamoto, M eng Comparative Study Research Support, Non-U.S. Gov't 1992/04/01 Mol Cell Biol. 1992 Apr; 12(4):1827-34. doi: 10.1128/mcb.12.4.1827-1834.1992"