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J Pharm Biomed Anal

Title:		Validation of a fast gas chromatographic method for the study of semiochemical slow release formulations
Author(s):		Heuskin S; Rozet E; Lorge S; Farmakidis J; Hubert P; Verheggen F; Haubruge E; Wathelet JP; Lognay G;
Address:		"Department of Analytical Chemistry, Gembloux Agro-Bio Tech, Liege University, Passage des Deportes 2, Gembloux, Belgium. stephanie.heuskin@ulg.ac.be"
Journal Title:		J Pharm Biomed Anal
Year:		2010
Volume:		20100722
Issue:		4
Page Number:		962 - 972
DOI:		10.1016/j.jpba.2010.07.014
ISSN/ISBN:		1873-264X (Electronic) 0731-7085 (Linking)
Abstract:		"The validation of a fast GC-FID analytical method for the quantitative determination of semiochemical sesquiterpenes (E-beta-farnesene and beta-caryophyllene) to be used in an integrated pest management approach is described. Accuracy profiles using total error as decision criteria for validation were used to verify the overall accuracy of the method results within a well defined range of concentrations and to determine the lowest limit of quantification for each analyte. Furthermore it allowed to select a very simple and reliable regression model for calibration curve for the quantification of both analytes as well as to provide measurement uncertainty without any additional experiments. Finally, this validated method was used for the quantification of semiochemicals in slow release formulations. The goal was to verify the protection efficiency of alginate gel beads formulations against oxidation and degradation of sesquiterpenes. The results showed that the alginate beads are adequate slow release devices which protect the bio-active molecules during at least twenty days"
Keywords:		"Chemistry, Pharmaceutical Chromatography, Gas/*methods Delayed-Action Preparations Flame Ionization Limit of Detection Sesquiterpenes/*analysis;"
Notes:		"MedlineHeuskin, Stephanie Rozet, Eric Lorge, Stephanie Farmakidis, Julien Hubert, Philippe Verheggen, Francois Haubruge, Eric Wathelet, Jean-Paul Lognay, Georges eng Research Support, Non-U.S. Gov't Validation Study England 2010/08/07 J Pharm Biomed Anal. 2010 Dec 1; 53(4):962-72. doi: 10.1016/j.jpba.2010.07.014. Epub 2010 Jul 22"