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Environ Sci Technol

Title:		Exposure to Dimethyl Selenide (DMSe)-Derived Secondary Organic Aerosol Alters Transcriptomic Profiles in Human Airway Epithelial Cells
Author(s):		Ahmed CMS; Cui Y; Frie AL; Burr A; Kamath R; Chen JY; Rahman A; Nordgren TM; Lin YH; Bahreini R;
Address:
Journal Title:		Environ Sci Technol
Year:		2019
Volume:		20191202
Issue:		24
Page Number:		14660 - 14669
DOI:		10.1021/acs.est.9b04376
ISSN/ISBN:		1520-5851 (Electronic) 0013-936X (Print) 0013-936X (Linking)
Abstract:		"Dimethyl selenide (DMSe) is one of the major volatile organoselenium compounds released from aquatic and terrestrial environments through microbial transformation and plant metabolism. The detailed processes of DMSe leading to secondary organic aerosol (SOA) formation and the pulmonary health effects induced by inhalation of DMSe-derived SOA remain largely unknown. In this study, we characterized the chemical composition and formation yields of SOA produced from the oxidation of DMSe with OH radicals and O(3) in controlled chamber experiments. Further, we profiled the transcriptome-wide gene expression changes in human airway epithelial cells (BEAS-2B) after exposure to DMSe-derived SOA. Our analyses indicated a significantly higher SOA yield resulting from the OH-initiated oxidation of DMSe. The oxidative potential of DMSe-derived SOA, as measured by the dithiothreitol (DTT) assay, suggested the presence of oxidizing moieties in DMSe-derived SOA at levels higher than typical ambient aerosols. Utilizing RNA sequencing (RNA-Seq) techniques, gene expression profiling followed by pathway enrichment analysis revealed several major biological pathways perturbed by DMSe-derived SOA, including elevated genotoxicity, DNA damage, and p53-mediated stress responses, as well as downregulated cholesterol biosynthesis, glycolysis, and interleukin IL-4/IL-13 signaling. This study highlights the significance of DMSe-derived SOA as a stressor in human airway epithelial cells"
Keywords:		Aerosols *Air Pollutants Epithelial Cells Humans *Organoselenium Compounds Oxidation-Reduction Transcriptome;
Notes:		"MedlineAhmed, C M Sabbir Cui, Yumeng Frie, Alexander L Burr, Abigail Kamath, Rohan Chen, Jin Y Rahman, Arafat Nordgren, Tara M Lin, Ying-Hsuan Bahreini, Roya eng R00 ES025819/ES/NIEHS NIH HHS/ S10 OD016290/OD/NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2019/11/22 Environ Sci Technol. 2019 Dec 17; 53(24):14660-14669. doi: 10.1021/acs.est.9b04376. Epub 2019 Dec 2"