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J Insect Sci


Title:"Preliminary analysis of several attractants and spatial repellents for the mosquito, Aedes albopictus using an olfactometer"
Author(s):Hao H; Sun J; Dai J;
Address:"Naval Medical Research Institute, 880 Xiangyin Road, Shanghai, 200433, China. haohuiling@hotmail.com"
Journal Title:J Insect Sci
Year:2012
Volume:12
Issue:
Page Number:76 -
DOI: 10.1673/031.012.7601
ISSN/ISBN:1536-2442 (Electronic) 1536-2442 (Linking)
Abstract:"Mosquito attractants and spatial repellents hold great promise in controlling mosquito pests. In assessing the effectiveness of mosquito attractants and repellents, a good olfactometer system, and optimized testing conditions, are essential. In this research, we demonstrated the usefulness of an olfactometer system, and optimized testing conditions for Aedes albopictus (Diptera: Culicidae). We found no significant difference in the biting activity of the insect between 8:00 and 22:00. Furthermore, 5-10 day old mosquitoes were relatively strongly attracted, with bloodsucking rate 75.8%. The random capture rate (entered trap in absence of odor) was less than 20% for the 20-40 tested insects. Capture rates and systematic errors increased as the number of testing insects increased. Wind speed affected the capture rate significantly, whereas variations in temperature between 25-28 degrees C did not result in significant difference. The wind speed of 0.2 m/s exhibited a higher capture rate, which was significantly different from those at either 0.1 m/s or 0.4 m/s (P < 0.05). At the wind speed of 0.2 m/s, time durations within the first 8 minutes correlated positively with capture rates (r(2) = 0.997), but further increase in time duration to 10 minutes did not result in further increase in capture rates. One percent of L-lactic acid in dichloromethane resulted in a consistently higher capture rate (43.8%) than that from a human odor (31.2%). Under our testing conditions, eugenol, anisaldehyde, geraniol, citronellal, citral, and linalool all exhibited some inhibition effect on mosquitoes to successfully trace human odor or 1% of L-lactic acid in dichloromethane. The results of these two tests indicate that the L-lactic acid/dichloromethane mixture may be used as an effective attractant to evaluate the effect of possible spatial repellents on Ae. albopictus"
Keywords:Aedes/*drug effects/physiology Animals Feeding Behavior Female Insect Control/*methods Insect Repellents/*pharmacology Pheromones/*pharmacology Smell;
Notes:"MedlineHao, Huiling Sun, Jingcheng Dai, Jianqing eng Evaluation Study 2013/02/20 J Insect Sci. 2012; 12:76. doi: 10.1673/031.012.7601"

 
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