| Title: | Pheromone discrimination by the pheromone-binding protein of Bombyx mori |
| Author(s): | Grater F; Xu W; Leal W; Grubmuller H; |
| Address: | "Department of Theoretical and Computational Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany" |
| DOI: | 10.1016/j.str.2006.08.013 |
| ISSN/ISBN: | 0969-2126 (Print) 0969-2126 (Linking) |
| Abstract: | "Pheromone-binding proteins are postulated to contribute to the exquisite specificity of the insect's olfactory system, acting as a filter by preferentially binding only one of the components of the natural pheromone. Here, we investigated the possible discrimination of the two very similar components of the natural pheromone gland from the silk moth, Bombyx mori, bombykol and bombykal, by the only pheromone-binding protein (BmorPBP) known to be expressed in the pheromone-detecting sensilla. Free-energy calculations and virtual docking indicate that both bombykol and bombykal bind to BmorPBP with similar affinity. In addition, in vitro competitive binding assays showed that both bombykol and bombykal were bound by BmorPBP with nearly the same high affinity. While BmorPBP might filter out other physiologically irrelevant compounds hitting the sensillar lymph, discrimination between the natural pheromone compounds must be achieved by molecular interactions with their cognate receptors" |
| Keywords: | "Alkadienes/chemistry Amino Acids/chemistry Animals Binding Sites Binding, Competitive Bombyx/*metabolism Carrier Proteins/*chemistry Computer Simulation Fatty Alcohols/chemistry Hydrogen Bonding Insect Proteins/*chemistry Intercellular Signaling Peptides;" |
| Notes: | "MedlineGrater, Frauke Xu, Wei Leal, Walter Grubmuller, Helmut eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2006/10/10 Structure. 2006 Oct; 14(10):1577-86. doi: 10.1016/j.str.2006.08.013" |
