| Title: | On-column solvent exchange for purified preparative fractions |
| Author(s): | Goetz GH; Beck E; Tidswell PW; |
| Address: | "Pfizer Global Research and Development, Analytical Chemistry and Sample Logistics, St Louis Laboratories, Chesterfield, MO, USA. gilles.h.goetz@pfizer.com" |
| DOI: | 10.1016/j.jala.2010.02.004 |
| ISSN/ISBN: | 2211-0690 (Electronic) 2211-0682 (Linking) |
| Abstract: | "On-column solvent exchange, using many of the principles of solid-phase extraction, has been implemented to significantly reduce evaporation cycle time following reverse-phase preparative HPLC. Additional benefits, such as a reduced potential for salt formation, thermal decomposition, and residual solvent, are also described. Fractions obtained from preparative separations, typically in a large volume of acetonitrile:water, are injected into the preparative HPLC and then eluted in acetonitrile, creating a new fraction in a volatile organic solvent. Minimal modification to the instrument was required, and unattended operation is possible. Acetonitrile evaporation is achieved within 3 h, compared with 17 h for aqueous-based fractions; lower temperatures can be used during the evaporation step; mobile-phase additives, likely to form salts with the target compound if concentrated in the fraction, are removed before evaporation; sample recovery and purity are unaffected" |
| Keywords: | "Acetonitriles/*chemistry Chromatography, High Pressure Liquid/*methods Solvents/*chemistry Volatilization;" |
| Notes: | "MedlineGoetz, Gilles H Beck, Emily Tidswell, Peter W eng 2011/09/13 J Lab Autom. 2011 Oct; 16(5):335-46. doi: 10.1016/j.jala.2010.02.004. Epub 2010 May 14" |
