| Title: | Trichoderma Histone Deacetylase HDA-2 Modulates Multiple Responses in Arabidopsis |
| Author(s): | Estrada-Rivera M; Rebolledo-Prudencio OG; Perez-Robles DA; Rocha-Medina MDC; Gonzalez-Lopez MDC; Casas-Flores S; |
| Address: | "Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Camino a la presa San Jose No. 2055, Colonia Lomas 4a seccion. C.P. 78216, San Luis Potosi, Mexico. Laboratorio Nacional de Biotecnologia Agricola, Medica y Ambiental, Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Camino a la presa San Jose No. 2055, Colonia Lomas 4a seccion. C.P. 78216, San Luis Potosi, Mexico. Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Camino a la presa San Jose No. 2055, Colonia Lomas 4a seccion. C.P. 78216, San Luis Potosi, Mexico scasas@ipicyt.edu.mx" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "Trichoderma spp. are a rich source of secondary metabolites and volatile organic compounds (VOCs), which may induce plant defenses and modulate plant growth. In filamentous fungi, chromatin modifications regulate secondary metabolism. In this study we investigated how the absence of histone deacetylase HDA-2 in the Trichoderma atroviride strain Deltahda-2 impacts its effect on a host, Arabidopsis (Arabidopsis thaliana). The production of VOCs and their impact on plant growth and development were assessed as well. The Deltahda-2 strain was impaired in its ability to colonize Arabidopsis roots, thus affecting the promotion of plant growth and modulation of plant defenses against foliar pathogens Botrytis cinerea and Pseudomonas syringae, which normally result from interaction with T. atroviride Furthermore, Deltahda-2 VOCs were incapable of triggering plant defenses to counterattack foliar pathogens. The Deltahda-2 overproduced the VOC 6-pentyl-2H-pyran-2-one (6-PP), which resulted in enhanced root branching and differentially regulated phytohormone-related genes. Analysis of ten VOCs (including 6-PP) revealed that three of them positively regulated plant growth, whereas six had the opposite effect. Assessment of secondary metabolites, detoxification, and communication with plant-related genes showed a dual role for HDA-2 in T. atroviride gene expression regulation during its interaction with plants. Chromatin immunoprecipitation of acetylated histone H3 on the promoters of plant-responsive genes in Deltahda-2 showed, in the presence of Arabidopsis, low levels of epl-1 and abc-2 compared with that in the wild type; whereas ctf-1 presented high constitutive levels, supporting a dual role of HDA-2 in gene regulation. This work highlights the importance of HDA-2 as a global regulator in Trichoderma to modulate multiple responses in Arabidopsis" |
| Keywords: | "Arabidopsis/growth & development/*microbiology Fungal Proteins/metabolism Gene Expression Regulation, Plant Histone Deacetylases/*metabolism Plant Development Plant Diseases Plant Immunity Plant Roots/growth & development/microbiology Trichoderma/*enzymol;" |
| Notes: | "MedlineEstrada-Rivera, Magnolia Rebolledo-Prudencio, Oscar Guillermo Perez-Robles, Doris Arisbeth Rocha-Medina, Ma Del Carmen Gonzalez-Lopez, Maria Del Carmen Casas-Flores, Sergio eng Research Support, Non-U.S. Gov't 2019/01/24 Plant Physiol. 2019 Apr; 179(4):1343-1361. doi: 10.1104/pp.18.01092. Epub 2019 Jan 22" |
