« Previous AbstractSurvey of volatile oxylipins and their biosynthetic precursors in bryophytes    Next AbstractA potential positive feedback loop controlling CLN1 and CLN2 gene expression at the start of the yeast cell cycle »

Environ Microbiol

Title:		"Regulation of plasmid-encoded isoprene metabolism in Rhodococcus, a representative of an important link in the global isoprene cycle"
Author(s):		Crombie AT; Khawand ME; Rhodius VA; Fengler KA; Miller MC; Whited GM; McGenity TJ; Murrell JC;
Address:		"University of East Anglia, Norwich Research Park, Norwich, UK. DuPont Industrial Biosciences, 925 Page Mill Road, Palo Alto, CA, 94304, USA. DuPont Pioneer, 7200 NW 62nd Avenue, Johnston, IA, 50131, USA. University of Essex, Wivenhoe Park, Colchester, UK"
Journal Title:		Environ Microbiol
Year:		2015
Volume:		20150415
Issue:		9
Page Number:		3314 - 3329
DOI:		10.1111/1462-2920.12793
ISSN/ISBN:		1462-2920 (Electronic) 1462-2912 (Print) 1462-2912 (Linking)
Abstract:		"Emissions of biogenic volatile organic compounds (VOCs) form an important part of the global carbon cycle, comprising a significant proportion of net ecosystem productivity. They impact atmospheric chemistry and contribute directly and indirectly to greenhouse gases. Isoprene, emitted largely from plants, comprises one third of total VOCs, yet in contrast to methane, which is released in similar quantities, we know little of its biodegradation. Here, we report the genome of an isoprene degrading isolate, Rhodococcus sp. AD45, and, using mutagenesis shows that a plasmid-encoded soluble di-iron centre isoprene monooxygenase (IsoMO) is essential for isoprene metabolism. Using RNA sequencing (RNAseq) to analyse cells exposed to isoprene or epoxyisoprene in a substrate-switch time-course experiment, we show that transcripts from 22 contiguous genes, including those encoding IsoMO, were highly upregulated, becoming among the most abundant in the cell and comprising over 25% of the entire transcriptome. Analysis of gene transcription in the wild type and an IsoMO-disrupted mutant strain showed that epoxyisoprene, or a subsequent product of isoprene metabolism, rather than isoprene itself, was the inducing molecule. We provide a foundation of molecular data for future research on the environmental biological consumption of this important, climate-active compound"
Keywords:		"Base Sequence Butadienes/*metabolism Carbon Cycle/*physiology Climate Ecosystem Gene Expression Profiling Genome, Bacterial Hemiterpenes/*metabolism Molecular Sequence Data Pentanes/*metabolism Plants/metabolism Plasmids/genetics RNA, Bacterial/genetics R;"
Notes:		"MedlineCrombie, Andrew T Khawand, Myriam El Rhodius, Virgil A Fengler, Kevin A Miller, Michael C Whited, Gregg M McGenity, Terry J Murrell, J Colin eng Research Support, Non-U.S. Gov't England 2015/03/03 Environ Microbiol. 2015 Sep; 17(9):3314-29. doi: 10.1111/1462-2920.12793. Epub 2015 Apr 15"